acid-phosphatase and Anaplasia

An immunoperoxidase study in 19 patients with cancer of prostate, using antiserums directed against prostatic acid phosphatase (PAP) and prostate specific antigen (PSA), allowed classification of tumors as strongly, moderately and poorly differentiated, and undifferentiated forms. Tests were conducted on specimens fixed and embedded in paraffin wax. Evaluation of degree of positivity for cells most marked by PAP and by PSA failed to establish its correlation with tumoral differentiation. Results, in fact, showed that poorly differentiated or undifferentiated forms of prostatic carcinoma contained cells with enhanced positive reactions when compared with strongly differentiated forms. Morphologically strongly differentiated forms showed weaker positivity than observed in normal prostate, this positivity being relatively homogeneous throughout the zone of proliferation, and cells varied little in their degree of positivity. Characteristic findings in less well differentiated forms were several strongly positive cells, with marked differences in positivity between cells and frequent alternating positive and negative zones. These findings may be of particular interest for diagnostic interpretation of strongly or poorly differentiated forms.

Topics: Acid Phosphatase; Adenocarcinoma; Anaplasia; Animals; Antigens, Neoplasm; Carcinoma; Diagnosis, Differential; Humans; Immune Sera; Immunoenzyme Techniques; Male; Prostate; Prostate-Specific Antigen; Prostatic Neoplasms; Rabbits

Twenty cases of acute lymphoblastic leukemia (ALL) in children and adults were investigated at different tissue localizations by scanning electron microscopy. ALL was divided into cases with or without strong paranuclear acid phosphatase activity. ALL showed very similar surface morphology irrespective of the type of ALL or the tissue localization. ALL is, however, strikingly different in some from other childhood leukemias and lymphomas, as well as from activated T-lymphocytes in infectious mononucleosis. The results indicate that the surface morphology of leukemic cells is a stable cytologic parameter, if certain technical prerequisits are fulfilled. Further criteria may thus be added to the panel of known cytologic, cytochemical and functional parameters.

Topics: Acid Phosphatase; Adult; Anaplasia; B-Lymphocytes; Cell Membrane; Child; Humans; Leukemia, Lymphoid; Lymphocytes; Microscopy, Electron, Scanning; T-Lymphocytes

We report on the development of the Dunning R3327 prostatic adenocarcinoma of the Copenhagen rat as a suitable model for human prostatic cancer. Tumors produced by the subcutaneous or intraprostatic injections of viable cells had the macroscopic and microscopic characteristics of human disease. Histologically, these tumors were well differentiated adenocarcinomas with the human disease. Histologically, these tumors were well differentiated adenocarcinomas with the formation of glands and acid secretions within the acini. The intraprostatic tumor, although initially confined to the injected lobe, grew to involve the surrounding tissues and eventually metastasized to the lymph nodes and lungs. Occasional metastatic lesions were found in other organs as well. Acid phosphatase could be demonstrated by histochemical staining of frozen tumor sections and elevated levels of the enzyme were seen in the serum of rats bearing long-term subcutaneous tumors. During investigation of the tumor a fast growing line arose that grew equally as well in female as in male rats. The histology of this tumor was of an undifferentiated anaplastic tumor. Treatment by cryosurgery completely destroyed the prostatic tumor within 2 weeks. A tissue culture line derived from R3327 was capable of producing tumors in recipient rats with characteristics similar to the original Dunning tumor.

Topics: Acid Phosphatase; Adenocarcinoma; Anaplasia; Animals; Female; Histocytochemistry; Male; Neoplasm Metastasis; Neoplasm Transplantation; Neoplasms, Experimental; Prostatic Neoplasms; Rats; Rats, Inbred Strains; Sex Factors

The Dunning R3327 prostate adenocarcinoma of the Copenhagen rat was developed as a suitable model of human prostate cancer. Inoculation of tumor tissue mince or cells sc in the flanks of recipient rats produced tumors that had the macroscopic and microscopic characteristics of the human disease. The histologic picture of these tumors was that of a well-differentiated adenocarcinoma with the formation of glands and acid secretions within the acini. Tumors were also produced in the dorsolateral lobe of the prostate by the injection of cells. The intraprostate tumor, although initially confined to the injected lobe, grew to involve the surrounding tissues and eventually metastasized to the lymph nodes and lungs. Occasional metastatic lesions were found in other organs also. Acid phosphatase could be domonstrated by histochemical staining of frozen tumor sections, and elevated levels of the enzyme were seen in the sera of rats bearing long-term subcutaneous tumors. During investigation of the tumor, a fast-growing tumor line arose that grew equally as well in females as in males. The histology of this tumor was that of an undifferentiated anaplastic tumor.

Topics: Acid Phosphatase; Adenocarcinoma; Anaplasia; Animals; Disease Models, Animal; Female; Male; Neoplasm Metastasis; Neoplasm Transplantation; Neoplasms, Experimental; Prostatic Neoplasms; Rats; Rats, Inbred Strains; Transplantation, Isogeneic