acid-phosphatase and Adenoma

This study was carried out in order to evaluate clinical usefulness of cross-linked N-telopeptides (NTx) of type I collagen determination, in patients with primary hyperparathyroidism. Twenty-six consecutive patients (6 males and 20 females, aged 56.3 +/- 15.0, SD, yrs) with primary hyperparathyroidism were studied in basal conditions and, ten of them, after surgical cure of the disease. Cross-linked collagen peptides were measured by enzyme-linked immunosorbent assay and conventional markers of bone turnover according to standard procedures. Bone densitometry at the lumbar spine and proximal femur was performed using dual-energy X-ray absorptiometry. Bone mineral density, was also assessed at the junction of the distal and middle third of the radius and at the ultradistal radius of the non-dominant arm by a dual photon densitometer. Mean urinary NTx values (194.2 +/- 121.9 pmoles bone collagen equivalents/mumoles creatinine) were significantly higher (p < 0.001) in respect to those found in normal subjects. The mean increase of Z score values of both serum tartrate resistant acid phosphatase activity (1.4 +/- 1.8) and the fasting hydroxyproline/creatinine ratio (1.45 +/- 2.0) was significantly lower (p < 0.02) in respect to that of NTx Z score values (3.3 +/- 3.3); the latter values were not significantly different than mean Z score values of serum osteocalcin (4.0 +/- 3.9), serum alkaline phosphatase activity (2.6 +/- 2.6) and urinary calcium/creatinine ratio (3.2 +/- 3.3). We found a significant inverse correlation between NTx values and both lumbar spine (p < 0.01) and ultradistal radius bone mineral density (p < 0.05); a modest inverse correlation was also observed between serum tartrate resistant acid phosphatase activity and lumbar spine bone mineral density (p < 0.04). Following successful adenoma removal, the percentage decrease of both NTx and hydroxyproline was similar in patients with increased bone turnover rate; major discrepancies were observed in patients with normal values of NTx, the telopeptide reduction being greater than that of hydroxyproline. Finally, in a hypercalcemic patient with metastatic parathyroid cancer, telopeptide excretion was shown to be more sensitive in respect to urinary hydroxyproline when evaluating the effects of antiresorptive therapy. Our results seem to indicate that amongst the markers with good sensitivity, NTx is the only one that is inversely related with bone mineral density at two different skeletal sites

Topics: Absorptiometry, Photon; Acid Phosphatase; Adenoma; Adult; Aged; Alkaline Phosphatase; Bone Density; Bone Resorption; Calcium; Collagen; Collagen Type I; Creatinine; Enzyme-Linked Immunosorbent Assay; Female; Humans; Hydroxyproline; Hyperparathyroidism; Male; Middle Aged; Osteocalcin; Parathyroid Neoplasms; Peptides; Tartrates

Phytochromes are ubiquitous red/far-red wavelength-sensitive photoreceptors in plants. Oat phytochrome A is a phosphoprotein. Phytochrome A (phyA) possesses two spatially different sites for phosphorylation with cAMP-dependent protein kinase (PKA) [McMichael & Lagarias (1990) Biochemistry 29, 3872-3878]. To assess the modulation of protein conformation by phosphorylation/dephosphorylation and its possible implication in phytochrome-mediated signal transduction, the conformations of phytochrome have been probed by PKA catalyzed phosphorylation. The phosphorylated species were purified and analyzed, along with untreated phytochrome, by limited proteolysis, circular dichroism (CD) and fluorescence quenching measurements. No significant changes in secondary structure of the phyA molecule after its phosphorylation were observed by CD. However, a subtle topographic and/or electrostatic effect of the phytochrome phosphorylation was detected by the time-resolved fluorescence quenching of Trp residues with Cs+ ions. N-Terminal phosphorylation at Ser17 was unique to the Pr form, but both Pr and Pfr phytochromes were phosphorylated at the hinge region to some extent. Phosphorylation at the hinge region resulted in noticeable changes in the proteolytic patterns, inhibiting cleavage near the phosphorylation site and favoring tryptic digestion of the Lys536-Asn537 peptide bond. Phosphorylation at the N-terminus did not cause observable changes in the helical structure of this region, but had an inhibitory effect on proteinase V8 accessibility at a site near the chromophore attachment. The functional relevance of protein phosphorylation of phyA is also discussed.

Topics: Acid Phosphatase; Adenoma; Adenosine Triphosphate; Amino Acid Sequence; Animals; Avena; Cattle; Circular Dichroism; Cyclic AMP-Dependent Protein Kinases; Humans; Kinetics; Male; Myocardium; Peptide Fragments; Peptide Mapping; Phosphorylation; Phosphoserine; Phosphotyrosine; Phytochrome; Phytochrome A; Prostate; Prostatic Neoplasms; Protein Conformation; Protein Structure, Secondary; Substrate Specificity; Trypsin

Ultrasound-guided ethanol injection (PEI) is an effective treatment of autonomously functioning thyroid nodules (AFTN) but little is known about the tissue lesions induced by alcohol. We report morphological changes of an AFTN which was removed after successful PEI treatment. At histological evaluation, the adenoma showed multiple areas of coagulative necrosis with eosinophilic ghost follicles, hemorrhages, small vessel thrombosis and a wedge-shaped hemorrhagic infarction. The peripheral adenomatous tissue and the surrounding thyroid parenchyma were free of regressive changes and/or lympho-monocytic inflammatory reactions. Enzyme histochemistry confirmed the hyperfunctioning character of the lesion and showed reduction of succinic dehydrogenase and cytochrome-c-oxydase activity in the viable tissue surrounding the central necrotic areas. By electron microscopy, the hyperfunctioning follicular epithelium showed submicroscopical alterations of the adenomatous cells surrounding the areas of coagulative necrosis. These results suggest that control of hyperthyroidism, after PEI, is due to: a) directly induced, irreversible damage (coagulative necrosis and vascular thrombosis with hemorrhagic infarction) in the central areas of the lesion; b) potentially reversible damage (reduction of intracellular enzyme activity and ultrastructural changes) in the peripheral areas.

Topics: Acid Phosphatase; Adenoma; Aged; Electron Transport Complex IV; Ethanol; Female; Histocytochemistry; Humans; Injections, Subcutaneous; Microscopy, Electron; Succinate Dehydrogenase; Thyroid Neoplasms

Bone loss and the serum markers of bone metabolism were studied in 22 patients with primary hyperparathyroidism and 108 patients with renal hyperparathyroidism. The parameters of bone loss were bone mineral density in the distal radius and lumbar vertebrae, measured by dual energy X-ray absorptiometry, and bone mass index (sigma GS/D) and the metacarpal index, in the second metacarpal bone, measured by the digital image processing method. Alkaline phosphatase (AIP), intact osteocalcin (OC), and the carboxyterminal propeptide of type I procollagen (PICP) were measured as serum markers of bone formation, while tartrate-resistant acid phosphatase (TRACP) and the carboxyterminal pyridinoline cross-linked telopeptide of type I collagen (ICTP) were measured as serum markers of bone resorption. Bone loss and elevated markers of bone metabolism were observed both in patients with skeletal symptoms and in those without. Furthermore, the decrease in the cortical bone mass was more predominant than that of the trabecular bone. As markers of bone formation, AIP and OC seemed to be more sensitive than PICP, and as markers of bone resorption, ICTP appeared to be more sensitive than TRACP. Thus, a close correlation was observed between bone loss and the markers of bone formation and resorption.

Topics: Acid Phosphatase; Adenoma; Alkaline Phosphatase; Biomarkers, Tumor; Bone and Bones; Bone Density; Bone Resorption; Chronic Kidney Disease-Mineral and Bone Disorder; Female; Humans; Hyperparathyroidism; Isoenzymes; Male; Middle Aged; Osteocalcin; Parathyroid Neoplasms; Procollagen; Tartrate-Resistant Acid Phosphatase

The authors are reporting the clinical importance of prostate specific antigen. They concluded that there are false positive and false negative cases. The prostate specific antigen is high in prostatic hyperplasia therefore the distinction between the two disease is impossible on basis of the prostate specific antigen. Prostate specific antigen shows the metastatic cases better [correction of worse] than prostatic acid phosphatase. But prostate specific antigen detects the changes of the clinical course of the disease well and shows the progression sooner. The authors have concluded that prostate specific antigen can not replace phosphatases and bone-scanning in the diagnosis and follow up of patients with prostate carcinoma.

Topics: Acid Phosphatase; Adenoma; Carcinoma; Diagnosis, Differential; False Positive Reactions; Humans; Male; Prostate-Specific Antigen; Prostatic Hyperplasia; Prostatic Neoplasms

The diagnostic value of the tumour markers: PSA, PAP and AcP was studied before treatment in 379 men (47 with prostatic cancer--PC, 306 with benign hyperplasia--PBH, and 26 healthy subjects--control group CG). PSA was determined by the enzymoimmune method, and the phosphatases were evaluated by the spectrophotometric method. Raised level of PSA was found in PBH--the highest value--23.3 ng/ml. After accepting the cutting off values (1.9 ng/ml and 23.3 ng/ml), even in 93% of patients with PC, the level of PSA exceeded the second of those values. A significant growing tendency was found of PSA together with the degree of clinical progression of PC (in stages C and D--in 100% of patients). PSA, as compared with the phosphatases, is a much more sensitive biochemical marker, exceeding them many times in sensitivity.

Topics: Acid Phosphatase; Adenoma; Aged; Aged, 80 and over; Biomarkers, Tumor; Humans; Male; Middle Aged; Prostate-Specific Antigen; Prostatic Neoplasms; Sensitivity and Specificity

We discuss a 63-year-old man who presented with a metastatic tumor in an inguinal lymph node. By light microscopy, the tumor cells were characterized by a finely granular eosinophilic cytoplasm. A diagnosis of metastatic oncocytic carcinoma was made based on the results of an ultrastructural examination, which showed the cytoplasm of the tumor cells to be filled with mitochondria. Results of immunocytochemical studies showed positive reactivity for prostatic acid phosphatase and prostate-specific antigen. A transurethral resection of the prostate showed an oncocytic adenocarcinoma of the prostate, apparently the first of its kind, which was demonstrated to be the site of origin of the inguinal lymph node metastasis.

Topics: Acid Phosphatase; Adenocarcinoma; Adenoma; Cell Transformation, Neoplastic; Diagnosis, Differential; Eosinophilia; Humans; Hyperplasia; Immunohistochemistry; Lymphatic Metastasis; Male; Microscopy, Electron; Middle Aged; Mitochondria; Prostate-Specific Antigen; Prostatic Neoplasms

The distribution of enzymes and laminin was examined in ileal tissue from pigs suffering from intestinal adenomatosis to reveal the nature of the lesion. A disruption of the normal and specific pattern of distribution was found. Thus, the normal ileal epithelium was characterised by brush border enzymes: alkaline phosphatase, magnesium-dependent adenosine triphosphatase (Mg-ATPase), fluoride resistant acid phosphatase and 5'-nucleotidase; enzymes of the basolateral border: Mg-ATPase; and cytoplasmic enzymes: beta-glucuronidase, non-specific esterase and acid phosphatase. Subepithelial fibroblasts seemed to be characterised by 5'-nucleotidase. Laminin was present as a continuous band under the surface and crypt epithelium, somewhat thicker in the former. In contrast, the branching proliferating crypts of intestinal adenomatosis largely lacked enzymes characteristic of both villus and crypt cells. Reactions for the subepithelial components, laminin and fibroblasts were also reduced. The deficient differentiation of the epithelial as well as subepithelial components in porcine intestinal adenomatosis distinguish the condition from crypt hyperplasia and indicate an adenoma-like character.

Topics: 5'-Nucleotidase; Acid Phosphatase; Adenoma; Alkaline Phosphatase; Animals; Ca(2+) Mg(2+)-ATPase; Carboxylesterase; Carboxylic Ester Hydrolases; Glucuronidase; Histocytochemistry; Ileum; Immunohistochemistry; Intestinal Neoplasms; Laminin; Swine; Swine Diseases

Testosterone-secreting adrenal adenoma is rare. We recently experienced a 17-year-old pubertal girl who showed signs of virilization and a high serum level of testosterone. The excised adrenal gland showed a 3.5 x 3 x 3-cm cortical adenoma. Light and electron microscopic findings together with the high serum level and high tumor tissue contents of testosterone and dehydroepiandrosterone (DHA) indicated that the tumor was a testosterone-secreting adrenal cortical adenoma. This appears to be a rather rare tumor from a review of the literature. Interestingly, in this case, the cytoplasm of the tumor cells contained structures resembling spironolactone bodies. From the results of enzyme histochemistry, the steroidogenetic pathways in this tumor were speculated.

Topics: Acid Phosphatase; Adenoma; Adolescent; Adrenal Cortex Neoplasms; Female; Histocytochemistry; Humans; Hydroxysteroid Dehydrogenases; Inclusion Bodies; Microscopy, Electron; Spironolactone; Testosterone

Altogether 184 patients with bone, renal or mixed hyperparathyroidism (HPT) presenting primarily were examined for blood levels of parathyroid hormone and indirect indicators of parathyroid function. None of the indices (calcium concentration, phosphorus level, serum activity of acid and alkaline phosphatase, oxyproline excretion with urine, etc.) was changed. The commonest biochemical signs of primary HPT were hypercalcemia, hypersecretion of parathyroid hormone, hyperoxyprolinuria. Biochemical indices deviations occurred more often and were more profound in bone primary HPT and parathyroid adenomas.

Topics: Acid Phosphatase; Adenoma; Alkaline Phosphatase; Calcium; Humans; Hydroxyproline; Hyperparathyroidism; Parathyroid Hormone; Parathyroid Neoplasms; Phosphorus; Radioimmunoassay

We report four cases of primary clear-cell adenoma and adenocarcinoma of the large intestine. The neoplasms grossly resembled ordinary colonic adenomas and adenocarcinomas but microscopically were composed of uniform cells with optically clear cytoplasm. Mucin stains were negative, and the clear nature of the cytoplasm was due to glycogen accumulation. Areas of transition between normal colonic epithelial constituents and the clear-cell lesion were observed. Three of the four cases stained strongly positively for carcinoembryonic antigen. These lesions are apt to give rise to considerable diagnostic confusion and, in particular, resemble metastatic renal cell carcinoma. The usual strong positive carcinoembryonic antigen reaction is helpful in establishing this diagnosis.

Topics: Acid Phosphatase; Adenocarcinoma; Adenoma; Adult; Aged; Carcinoembryonic Antigen; Colonic Neoplasms; Female; Humans; Immunoenzyme Techniques; Male; Membrane Glycoproteins; Middle Aged; Mucin-1

The monomorphic basal cell adenoma of parotid gland is a rare tumor of salivary glands. Its diagnosis is difficult with adenoid cystic carcinoma. In the case reported here, a peculiar type named membranous adenoma, the thickness of basement membranes, their penetration inside the epithelial lobules, form true cylinders as in adenoid cystic carcinoma. The histoenzymology and the electron microscopy provide important diagnostic arguments: activity of alkaline phosphatases lower than in cystic adenoid carcinoma; cells of adenoma more differentiated (epidermoid or secretory cells) than those of cystic adenoid carcinoma. The prognosis of the tumor is good, though recurrences may occur.

Topics: Acid Phosphatase; Adenoma; Adult; Alkaline Phosphatase; Epithelium; Female; Histocytochemistry; Humans; Microscopy, Electron; Oxidoreductases; Parotid Neoplasms

Enzyme histo-cytochemical staining was performed for 3 beta-hydroxysteroid dehydrogenase, alkaline phosphatase and acid phosphatase in the compact and clear cells of the adrenocortical adenomas associated with Cushing's syndrome (Cushing's adenomas). In the compact cells, enzymatic activities of 3 beta-hydroxysteroid dehydrogenase and alkaline phosphatase were stronger than those in the clear cells. Electron microscopic localization of alkaline phosphatase was mostly present on well developed microvilli, while, acid phosphatase was observed in lysosomes near the well developed smooth endoplasmic reticula. On the other hand, in the clear cells, cytochemical localization of alkaline phosphatase was mainly present on the intercellular plasma membrane, while acid phosphatase was mostly observed near the lipid droplets. Based on these data, functional aspects of the compact and clear cells in Cushing's adenomas are discussed.

Topics: 3-Hydroxysteroid Dehydrogenases; Acid Phosphatase; Adenoma; Adrenal Cortex Neoplasms; Adrenocorticotropic Hormone; Adult; Alkaline Phosphatase; Cushing Syndrome; Female; Histocytochemistry; Humans; Middle Aged

Two types of salivary monomorphic adenomas, the so-called adenolymphoma and oncocytoma (75 cases in a series of 873 salivary gland tumors) were studied. These tumors were almost always located in major salivary glands (essentially in the parotid gland). They were much more common in men (85%) than in women. The oncocyte represented the characteristic cell in these two neoplasms. By electron microscopy, they were seen to contain numerous and abnormal mitochondriae and well-developed lysosomal systems. These findings were correlated with a high level of activity of oxidative enzymes and of acid phosphatases. The histogenesis of these tumors was discussed. They seemed to arise from aberrant striated ducts embedded in heterotopic lymph nodes. The tumoral oncocytes would suffer a primary disturbance of their oxidative metabolism followed by a compensative mitochondrial hypertrophy.

Topics: Acid Phosphatase; Adenolymphoma; Adenoma; Female; Humans; Male; Microscopy, Electron; Salivary Gland Neoplasms

Enzyme histocytochemical staining including alkaline phosphatase, acid phosphatase, 3 beta hydroxysteroid dehydrogenase and 11 beta hydroxysteroid dehydrogenase was studied in human adrenocortical adenomas associated with primary aldosteronism. Histochemically, these enzyme activities were weaker in clear cell type adenomas than in the normal human adrenal cortex. While compact cell type adenomas showed stronger enzyme activities than the clear cell type adenomas. Electron microscopic localization of alkaline phosphatase was mainly observed in plasma membrane at the intercellular sites. Occasionally, this enzyme activity was also observed in the mitochondrial outer membrane, mainly in compact cells. The significance of these enzymes in the adenoma cells is discussed.

Topics: 3-Hydroxysteroid Dehydrogenases; Acid Phosphatase; Adenoma; Adrenal Cortex Neoplasms; Adult; Alkaline Phosphatase; Female; Histocytochemistry; Humans; Hyperaldosteronism; Male; Middle Aged

Topics: Acid Phosphatase; Adenoma; Animals; Humans; Immunologic Techniques; Male; Prostatic Neoplasms; Rabbits

Prostatic acid phosphatase was purified from prostatic adenomas. The procedure involved chromatography on Concanavalin A-Sepharose, DEAE-cellulose, Bio-Gel P-150 and L-tartrate-Sepharose. The purified phosphatase hydrolyzed p-nitrophenyl phosphate at a rate of 270 mumol . mg-1 . min-1 (25 degrees C) and showed homogeneity upon polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The final prostatic acid phosphatase preparation was pure and the antisera were monospecific as judged by the highly sensitive technique of crossed immunoelectrophoresis. Of the procedures evaluated for the iodination of the purified enzyme, oxidation with Iodogen was found to give the best iodinated product.

Topics: Acid Phosphatase; Adenoma; Chromatography; Drug Stability; Humans; Imidazoles; Immunoelectrophoresis, Two-Dimensional; Indicators and Reagents; Iodine Radioisotopes; Male; Molecular Weight; Oxidation-Reduction; Prostatic Neoplasms; Urea

Radioimmunological estimation of prostatic acid phosphatase was carried out in 72 reference subjects, 46 patients with benign prostatic hypertrophy, 106 patients with untreated prostatic carcinoma and 25 patients with a carcinoma of some other origin. The mean concentration in non-acidified serum was 1.3 +/- 0.4 (M +/- SD) ng/ml for the reference group and 1.6 +/- 0.8 ng/ml for the benign hypertrophy group. The upper limit of discriminatory values for the diagnosis of prostatic carcinoma was fixed at 3 ng/ml. Taking this value, the overall percentage of positive results for carcinoma of the prostate was 61% (65/106). The number of cases with a value greater than 3 ng/ml was 3/18 (17%) for stage A, 8/27 (30%) for stage B, 7/13 (54%) for stage C and 47/48 (98%) for stage D. 8% (2/25) of carcinomas of another origin gave a positive result. The results of estimation using the radioimmunological technique were compared with those obtained by the measurement of enzyme activity using para nitro-phenyl phosphate as a substrate in 34 untreated prostatic carcinomas (all stages mixed together). When measurements by both techniques were carried out under the same ideal conditions using fresh sera as soon as possible after the blood was drawn, the result was abnormal in 10 cases out of 12 (83%) for the radioimmunological method and in 8 cases out of 12 (67%) for the measurement of enzyme activity. By contrast, under routine conditions, the positive percentage figures were 77% (17/22) for the radioimmunological technique and only 36% (8/22) for the measurement of enzyme activity. It would thus appear that radioimmunological measurement is more reliable than the measurement of enzyme activity.

Topics: Acid Phosphatase; Adenoma; Clinical Enzyme Tests; Humans; Male; Prostate; Prostatic Neoplasms; Radioimmunoassay

The activity of acid phosphatase in thyroid cells obtained by aspiration biopsy with thin needle was studied. The results were compared with those, found by a biochemical method in the operation material of the same cases. It has been proved that there is a significant difference between malignant tumours with cytochemically mostly positive acid phosphatase reaction and colloid adenomas with mostly negative reaction. The results obtained by biochemical method showed significant quantitative differences between malignant and benign tumours. The pattern of acid phosphatase subunits (isoenzymes) in the sera of patients with thyroid malignancies were pathologic and similar to those found in mammary cancers. It can be supposed that the behavior of acid phosphatase in thyreocytes depends on same metabolic properties, perhaps on some process characteristic for malignancy, e.g. growth activity. Even this finding of cytochemically positive acid phosphatase reaction cannot be awaited to be quite specific, it has proved as a useful marker signalizing the possibility of malignancy.

Topics: Acid Phosphatase; Adenoma; Biopsy, Needle; Goiter; Humans; Hydrogen-Ion Concentration; Thyroid Neoplasms; Thyroiditis

Combined immunohistochemical staining (IHCS) and enzyme histochemical staining (EHCS) methods for light microscopy (LM) and electron microscopy (EM) are reported, using oestrogen-induced rat pituitary tumours. For LM, combined staining for alkaline phosphatase and acid phosphatase by EHCS, using the azo dye method, and for prolactin and ACTH by IHCS, using the enzyme-labelled antibody method, gave the best results on 1 microgram glycol methacrylate sections. For EM, combined staining by EHCS on 30 microgram tissue sections followed by IHCS for prolactin on ultrathin Epon sections (enzyme-labelled antibody method) provided acceptable results. By these combined staining methods, the neoplastic prolactin cells were shown to have close affinity to rich alkaline phosphatase-positive capillaries and to possess an alkaline phosphatase-positive cell membrane. Furthermore, they revealed acid phosphatase-positive lysosomal and secretory granules. These combined staining methods may be valuable in studies on the actual functional status of cells.

Topics: Acid Phosphatase; Adenoma; Adrenocorticotropic Hormone; Alkaline Phosphatase; Animals; Female; Histocytochemistry; Immunoenzyme Techniques; Microscopy, Electron; Neoplasms, Experimental; Pituitary Neoplasms; Prolactin; Rats

Ten weekly doses of dimethylhydrazine (30 mg/kg) were given to rats to induce colonic tumors. Histochemical and electron cytochemical studies revealed a distinct pattern of lysosomal acid phosphatase and beta-glucuronidase activity in macrophages in the stroma of these neoplasms. A dramatic increase in the number of acid phosphatase-rich macrophages was present in adenomas when compared to that in normal colonic mucosa. Fewer numbers of these cells were seen in well-differentiated adenocarcinomas, and they were barely detectable in highly invasive mucinous adenocarcinomas. It is postulated that these macrophages may play a role in preventing the invasion of adenomatous neoplasms into the submucosa. Application of histochemical techniques to study macrophage lysosomal enzymes may prove a useful diagnostic tool in differentiation of human colonic tumors for prognostic evaluation.

Topics: Acid Phosphatase; Adenocarcinoma; Adenocarcinoma, Mucinous; Adenoma; Animals; Colonic Neoplasms; Dimethylhydrazines; Glucuronidase; Lysosomes; Macrophages; Male; Methylhydrazines; Neoplasms, Experimental; Rats

Proliferation of the C cells of the rat thyroid gland under conditions of prolonged hypercalcemia induced with vit. D3 leading sometimes to a formation of adenoma-like nodules was observed. Metaplasia of the follicular epithelium into the C cells observed in the process of nodules formation seems to point out that the C cells and the follicular cells of the thyroid gland arise from a common maternal cell.

Topics: Acid Phosphatase; Adenoma; Animals; Cell Membrane; Male; Rats; Thyroid Gland; Thyroid Neoplasms

Prostatic infarction is a relatively frequent complication of adenoma of the bladder neck. Nevertheless its importance is minimal as compared with infarctions of vital organs like the brain, heart, lungs or kidneys. General and local factors may play a role in its pathogenesis. Besides other factors, it may contribute to the development of acute retention. Attention is called to the difficulties of differential diagnosis at both gross and microscopic examinations. A misdiagnosis is most frequently made in neoplastic diseases of the prostate.

Topics: Acid Phosphatase; Adenoma; Aged; Diagnosis, Differential; Humans; Infarction; Male; Middle Aged; Prostate; Urinary Bladder Neoplasms; Urination Disorders

Topics: Acid Phosphatase; Adenoma; Adult; Female; Humans; Microscopy, Electron; Middle Aged; NAD; Phosphorylases; Succinate Dehydrogenase; Sweat Gland Neoplasms

Topics: Acid Phosphatase; Adenoma; Carcinoma; Cytoplasm; Esterases; Histocytochemistry; Humans; Iodides; Iodine Radioisotopes; Microscopy, Electron; Mitochondria; Peroxidases; Subcellular Fractions; Thyroglobulin; Thyroid Neoplasms

Acid-phosphatase-rich granules, separate from the parathyroid-hormone-containing secretory granules, have been demonstrated in the normal, adenomatous and primary hyperplastic human parathyroid glands. These granules are thought to represent lysosomes which are derived from the Golgi region. In the normal glands these granules are postulated to be associated with involution of the cell organelles during the normal secretory cycle. In the adenomatous and hyperplastic glands evidence is presented that these lysosomes are associated with the destruction of secretary granules and parthyroid hormone.

Topics: Acid Phosphatase; Adenoma; Extracellular Space; Golgi Apparatus; Humans; Hyperplasia; Lysosomes; Parathyroid Diseases; Parathyroid Glands; Parathyroid Neoplasms

Topics: Acid Phosphatase; Adenocarcinoma; Adenoma; Adenosine Triphosphatases; Adult; Aged; Alkaline Phosphatase; Deoxyribonucleases; Dihydrolipoamide Dehydrogenase; DNA, Neoplasm; Electron Transport Complex IV; Female; Glucosephosphate Dehydrogenase; Glycosaminoglycans; Histocytochemistry; Humans; Malate Dehydrogenase; Male; Middle Aged; Nucleotidases; Ribonucleases; RNA, Neoplasm; Succinate Dehydrogenase; Urinary Bladder Neoplasms

Topics: Acid Phosphatase; Adenoma; Adenosine Triphosphatases; Alkaline Phosphatase; Aminopeptidases; Bronchial Neoplasms; Carcinoma, Adenoid Cystic; Carcinoma, Bronchogenic; Clinical Enzyme Tests; Diagnosis, Differential; Dihydrolipoamide Dehydrogenase; Esterases; Glucosephosphate Dehydrogenase; Glutamate Dehydrogenase; Glycerolphosphate Dehydrogenase; Hamartoma; Histocytochemistry; Humans; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Leiomyoma; Lung Neoplasms; Malate Dehydrogenase; Nucleotidases; Phosphogluconate Dehydrogenase; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenoma; Endoplasmic Reticulum; Golgi Apparatus; Histocytochemistry; Humans; Lysosomes; Microscopy, Electron; Pituitary Neoplasms

Topics: Acid Phosphatase; Adenoma; Adenosine Triphosphatases; Aged; Female; Glucosephosphate Dehydrogenase; Glucosyltransferases; Glycogen; Glycogen Storage Disease; Humans; Leucyl Aminopeptidase; Microscopy, Electron; Nucleotidases; Phosphotransferases; Polysaccharides; Skin; Succinate Dehydrogenase; Sweat Gland Neoplasms

Topics: Acid Phosphatase; Adenoma; Adult; Alkaline Phosphatase; Chromatography, Thin Layer; Edetic Acid; Electrophoresis; Epithelium; Female; Gels; Humans; Hydrogen-Ion Concentration; Hyperparathyroidism; Isoenzymes; Middle Aged; Parathyroid Neoplasms; Starch; Tartrates

Topics: Acid Phosphatase; Adenocarcinoma, Mucinous; Adenoma; Adenoma, Pleomorphic; Adenosine Triphosphatases; Adult; Aged; Alkaline Phosphatase; Carcinoma, Adenoid Cystic; Cystadenoma; Dihydrolipoamide Dehydrogenase; Female; Glucosephosphate Dehydrogenase; Histocytochemistry; Humans; Leucyl Aminopeptidase; Male; Middle Aged; Monoamine Oxidase; Parotid Neoplasms; Salivary Gland Neoplasms; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenoma; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Esterases; Glucose-6-Phosphatase; Glucosephosphate Dehydrogenase; Hydroxybutyrate Dehydrogenase; Kidney Neoplasms; Kidney Tubules; L-Lactate Dehydrogenase; Nitrosamines; Nucleotidases; Rats; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenoma; Animals; Cytoplasmic Granules; D-Amino-Acid Oxidase; Dog Diseases; Dogs; Female; Histocytochemistry; Male; Microscopy, Electron; Neoplasms; Oxidoreductases; Perianal Glands; Urate Oxidase

Topics: Acid Phosphatase; Adenoma; Aged; Alkaline Phosphatase; Bilirubin; Blood Coagulation Disorders; Blood Coagulation Tests; Blood Protein Electrophoresis; Blood Proteins; Blood Sedimentation; Bone and Bones; Heparin; Humans; Lung; Lymphatic Metastasis; Male; Neoplasm Metastasis; Prostatectomy; Prostatic Neoplasms; Thrombosis; Urea

Topics: Acid Phosphatase; Adenoma; Adenosine Triphosphatases; Histocytochemistry; Humans; Male; Middle Aged; Salivary Glands; Tongue Neoplasms

Topics: Acid Phosphatase; Adenoma; Animals; Golgi Apparatus; Histocytochemistry; Kidney Neoplasms; Lysosomes; Neoplasms, Experimental; Organoids; Rats

Topics: Acid Phosphatase; Adenoma; Brain Abscess; Brain Neoplasms; Cerebral Cortex; Ependymoma; Glioma; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Medulloblastoma; Meningioma; Microscopy, Fluorescence; Pituitary Neoplasms; Staining and Labeling

Topics: Acid Phosphatase; Adenoma; Alkaline Phosphatase; Ameloblastoma; Arthritis; Bone Cysts; Bone Neoplasms; Chondrosarcoma; Fibroma; Fibrous Dysplasia of Bone; Geriatrics; Giant Cell Tumors; Humans; Neoplasm Metastasis; Osteitis Fibrosa Cystica; Osteoma; Osteosarcoma; Prognosis; Radiography; Sarcoma, Ewing; Tuberculosis; Tuberculosis, Osteoarticular

Topics: Acid Phosphatase; Adenoma; Adrenal Cortex; Adrenal Gland Diseases; Adrenal Gland Neoplasms; Adrenal Glands; Adrenocortical Hyperfunction; Esterases; Geriatrics; Histocytochemistry; Humans; Hyperplasia; Hypertrophy; Lipid Metabolism