acid-phosphatase and Adenofibroma

Topics: Acid Phosphatase; Adenofibroma; Alkaline Phosphatase; Breast; Breast Neoplasms; Female; Glucosephosphate Dehydrogenase; Humans

Enzyme histochemical investigations of prostatic carcinoma were done by means of reference enzymes of different metabolic pathways. The results of these investigations show, that the loss of typical enzymatic design is not depending on histological grade of tumor differentiation. On the contrary each prostatic carcinoma represents a heterogenic population without any specificity. The morphological pattern alone does not allow conclusions as to degree of malignancy.

Topics: Acid Phosphatase; Adenocarcinoma; Adenofibroma; Adenosine Triphosphatases; Alkaline Phosphatase; Carcinoma; Glucose-6-Phosphatase; Glucosephosphate Dehydrogenase; Histocytochemistry; Humans; Hydrolases; L-Iditol 2-Dehydrogenase; Malate Dehydrogenase; Male; Nucleotidases; Oxidoreductases; Prostatic Neoplasms; Succinate Dehydrogenase

An ultramicrochemical technique has been adapted to the evolution of enzyme profiles within individual human mammary tumors. Tandem observation of adjacent stained and lyophilized sections permitted dissection of microgram quantities of freeze-dried material within confirmed regions of malignancy. Enzymes frequently monitored to examine glycolytic, respiratory, and metastatic capacity were microanalyzed successfully: lactic dehydrogenase (LDH), phosphoglucose isomerase (PGI), malate dehydrogenase (MDH), acid phosphatase (AP), aldolase (ALD), glucose-6-phosphate dehydrogenase (G6PDH), pyruvate kinase (PK), alpha-glycerophosphate dehydrogenase (alpha-GOPDH), hexokinase (HK), and phosphofructokinase (PRK). All enzyme activities were higher in infiltrating ductal carcinomas than in fibroadenomas. Extracts of tumor cells mixed in varying proportions with brain or muscle extracts of rat evidenced no modification of expected activity. The technical adaptation described provided a sensitive methodology to resolve problems of relication, profile analysis, sample quantity, and selectivity within heterogeneous tissues.

Topics: Acid Phosphatase; Adenofibroma; Alcohol Oxidoreductases; Breast Neoplasms; Carcinoma, Intraductal, Noninfiltrating; Female; Fructose-Bisphosphate Aldolase; Glucose-6-Phosphate Isomerase; Humans; Microchemistry; Phosphotransferases

Topics: Acid Phosphatase; Adenofibroma; Alkaline Phosphatase; Breast Neoplasms; Histocytochemistry; Humans; Papilloma

The synovial sheath obtained in synovectomy in 35 patients with rheumatic- and rheumatic-visceral forms of rheumatoid arthritis was studied histochemically and immunomorphologically. At early stages of exacerbation of the pathological process in the synovial tissue there were revealed predominantly catabolic processes: an increased permeability of vessels; mucoid oedema; fibrinoid changes in the subintimal layer. Further development of the disease was characterized by predominance of anabolic processes with proliferation of synoviocytes, subintimal histiocytes, productive vasculites, massive lymphoid-plasmocytic infiltration, diffuse, or in the form of lymphoid follicles. Using the immunofluorescent technique the authors revealed luminescence of the rheumatoid factor and gamma=globulin in plasmatic cells, extracellularly, and more rarely in macrophages. Pronounced immunological changes in the synovial sheath in the active course of rheumatoid arthritis were accompanied by a high level of metabolic processes and an intensive phagocytic reaction in synoviocytes and subintimal histiocytes. In observations with a low activity of rheumatoid arthritis the synovial tissue was characterized by low levels of enzymes of oxidative metabolism and hydrolysis, emptying of the capillary bed, processes of sclerosis, hyalinosis, amyloidosis.

Topics: Acid Phosphatase; Adenofibroma; Adult; Aged; Alkaline Phosphatase; Arthritis, Rheumatoid; Capillary Permeability; Chronic Disease; Dihydrolipoamide Dehydrogenase; Female; Fluorescent Antibody Technique; gamma-Globulins; Glucosephosphate Dehydrogenase; Glycerolphosphate Dehydrogenase; Glycosaminoglycans; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Male; Middle Aged; Plasma Cells; Radiography; Rheumatoid Factor; Synovial Membrane; Synovitis

Histochemical studies of human breast tumors were performed with particular emphasis on the activity of alkaline phosphatase (AIP), acid phosphatase (AcP) and glucose-6-phosphate dehydrogenase (G6PDH). Enzyme activities in benign and malignant lesions were compared. AIP was prominent in normal mammary epithelium, limited to the myoepithelial layer in benign tumors and was absent in cords of malignant cells. AcP activity was faintly detected in normal mammary epithelium, increased in canalicular epithelium of fibroadenomas and was marked in malignant cells. G6PDH exhibited marked activity in neoplastic epithelium and the stroma of nearly all carcinomas studied, whereas in benign tumors, G6PDH activity was strictly limited to the connective tissue. The study suggests a strong correlation between G6PDH activity and malignancy. The different results obtained by various workers in this field are critically reviewed, and discussed in the light of the results of the present study.

Topics: Acid Phosphatase; Adenofibroma; Adolescent; Adult; Aged; Alkaline Phosphatase; Biopsy; Breast; Breast Neoplasms; Carcinoma; Epithelial Cells; Epithelium; Female; Glucosephosphate Dehydrogenase; Histocytochemistry; Humans; Lactation; Middle Aged; Pregnancy

Topics: Acid Phosphatase; Adenofibroma; Adult; Aged; Alkaline Phosphatase; Biopsy; Breast Diseases; Breast Neoplasms; Capillaries; Cell Division; Epithelial Cells; Epithelium; Female; Fibroblasts; Glucosephosphate Dehydrogenase; Glycerolphosphate Dehydrogenase; Gynecomastia; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Male; Middle Aged; NADH, NADPH Oxidoreductases; Oxidoreductases; Staining and Labeling; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenofibroma; Breast; Breast Diseases; Breast Neoplasms; Cysts; Epithelium; Esterases; Female; Histocytochemistry; Humans; Methods; Staining and Labeling; Ubiquinone

Topics: Acid Phosphatase; Adenocarcinoma; Adenofibroma; Aged; Biopsy; Carcinoma; Diagnosis, Differential; Glucosephosphate Dehydrogenase Deficiency; Glutamate Dehydrogenase; Histocytochemistry; Humans; Hydroxybutyrate Dehydrogenase; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Male; Middle Aged; Prostatic Neoplasms; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenofibroma; Adult; Aged; Alkaline Phosphatase; Breast Neoplasms; Dihydrolipoamide Dehydrogenase; Female; Glucosephosphate Dehydrogenase; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Middle Aged; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenofibroma; Alkaline Phosphatase; Ameloblastoma; Aminopeptidases; Animals; Breast Neoplasms; Carcinoma, Squamous Cell; Female; Glucosephosphate Dehydrogenase; Glucuronidase; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Malate Dehydrogenase; Male; Mice; Mouth Neoplasms; Sarcoma, Experimental; Skin Neoplasms; Succinate Dehydrogenase; Tumor Virus Infections