acetylcholine-receptor-alpha-subunit-(125-148) and Thymoma

acetylcholine-receptor-alpha-subunit-(125-148) has been researched along with Thymoma* in 2 studies

Other Studies

2 other study(ies) available for acetylcholine-receptor-alpha-subunit-(125-148) and Thymoma

ArticleYear
Cloning of a cDNA coding for the acetylcholine receptor alpha-subunit from a thymoma associated with myasthenia [correction of myastenia] gravis.
    Thymus, 1994, Volume: 23, Issue:2

    To investigate the role of the acetylcholine receptor (AchR) in the pathogenesis of paraneoplastic Myasthenia gravis (MG), we screened a cDNA library of a MG-associated thymoma with a DNA oligonucleotide coding for aa 371-378, i.e. for part of the very immunogenic cytoplasmatic epitope (VICE-alpha, aa 373-380) of the human AChR alpha-subunit. We isolated two cDNA clones. Analysis of these clones has identified an open reading frame of 1371 bp, coding for the AChR alpha-subunit. No point mutation, insertion or deletion could be detected. Since the thymoma did not contain thymic myoid cells, which normally express AChR, the origin of the AChR transcripts must be the tumor cells itself. These findings confirm former results, where AChR alpha-subunit sequences from MG-thymomas were amplified by PCR.

    Topics: Base Sequence; Blotting, Northern; Cloning, Molecular; DNA, Complementary; Humans; Immunohistochemistry; Molecular Sequence Data; Myasthenia Gravis; Peptide Fragments; Receptors, Nicotinic; RNA, Messenger; Thymoma

1994
Antibodies to synthetic peptide (125-148) of the alpha-subunit of human nicotinic acetylcholine receptor in sera from patients with myasthenia gravis.
    Neurology, 1990, Volume: 40, Issue:11

    We measured the amount of antibodies to a synthetic peptide that corresponds to the alpha-subunit residues Lys125-Thr148 of human acetylcholine receptor (AChR) in myasthenic sera. We detected anti-peptide antibodies in 52% (89/171) of the patients with myasthenia gravis (MG), but none in any of the healthy controls. Anti-peptide antibodies should provide a valuable immunologic parameter for the clinical evaluation of MG, but no apparent correlation was observed between the titers of anti-peptide and anti-AChR antibodies.

    Topics: Autoantibodies; Humans; Myasthenia Gravis; Peptide Fragments; Receptors, Nicotinic; Thymoma; Thymus Neoplasms

1990