acetylcellulose and Zika-Virus-Infection

Many serious public health emergencies around the globe are caused by viral epidemics. Thus, developing a reliable method for viral screening is in high demand. Multiplex assays for simultaneous detection and fast screening of high-risk pathogens are especially needed. This study employs metal nanoparticles to generate specific mass spectral signals for different RNA viruses, which enables simultaneous detection of whole viruses by laser desorption/ionization mass spectrometry (LDI-MS). We developed a nanoparticle-based sandwich immunosorbent assay as a sensing platform for the detection of viruses and viral nonstructural protein by LDI-MS. Cellulose acetate membrane (CAM) serves as the substrate for the fabrication of the sandwich immunosorbent assay with the advantages of clean mass spectra and high enrichment of analytes. Antibody-modified metal nanoparticles (Ab-MNPs; M = Au or Ag) act as metallic biocodes for the LDI-MS detection. The signal amplification readout for the virus is through the pulsed laser-induced formation of metal cluster ions ([M

Topics: Adult; Animals; Antibodies, Immobilized; Antibodies, Monoclonal; Blood; Cellulose; Encephalitis Virus, Japanese; Encephalitis, Japanese; Enterovirus A, Human; Flavivirus Infections; Humans; Immunoassay; Limit of Detection; Male; Mass Spectrometry; Membranes, Artificial; Metal Nanoparticles; Mice; RNA Viruses; Viral Nonstructural Proteins; Young Adult; Zika Virus; Zika Virus Infection