acetyl-aspartyl-glutamyl-valyl-aspartal and Teratocarcinoma

Hinokitiol, a potent iron chelator, has been reported to induce differentiation in teratocarcinoma F9 cells with a reduction of viable cells. In this study, we examined the steps leading to eventual cell death by hinokitiol during differentiation. Hinokitiol induced DNA fragmentation of F9 cells in a concentration- and time-dependent manner. This effect was also observed in a cell-free system using the nuclei from intact cells and the cytosols from hinokitiol-treated cells. In contrast, hinokitiol methyl ether and hinokitiol-Fe (III) complex, which are deficient in iron-chelating activity, showed no DNA fragmentation activity in both cell culture and cell-free systems. These results suggest that iron deprivation by hinokitiol may be involved in the induction of apoptosis of F9 cells. Caspase-3, one of the key enzymes in the apoptotic cascade, was specifically activated by hinokitiol treatment, but not by the other two derivatives. In addition, its specific inhibitor, benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone, strongly blocked hinokitiol-induced DNA fragmentation. These results indicate that iron deprivation by hinokitiol can induce apoptosis of F9 cells through the activation of caspase-3.

Topics: Amino Acid Chloromethyl Ketones; Aniline Compounds; Apoptosis; Caspase 3; Caspases; Cell-Free System; Cysteine Proteinase Inhibitors; DNA Fragmentation; Embryonal Carcinoma Stem Cells; Enzyme Activation; Iron Chelating Agents; Monoterpenes; Neoplastic Stem Cells; Oligopeptides; Teratocarcinoma; Tropolone