a-967079 has been researched along with Adenocarcinoma* in 1 studies
1 other study(ies) available for a-967079 and Adenocarcinoma
Article | Year |
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Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells.
The transient receptor potential ankyrin type 1 (TRPA1) channel belongs to the TRP superfamily of ion channels. TRPA1 is a membrane protein with multiple functions able to respond to noxious stimuli, reactive oxygen species, inflammatory cytokines or pungent substances, and it participates in pain signalling, taste, inflammation and various steps of the tumorigenic process. To date, no reports have addressed the expression and function of TRPA1 in pancreatic ductal adenocarcinoma (PDAC) cells. This work reports the endogenous expression of TRPA1 channels in human pancreatic adenocarcinoma cell lines and provides insights into the function of the TRPA1 protein in the Panc-1 cell line. This study reports that cell lines isolated from PDAC patients had different levels of TRPA1 expression. The channel activity in Panc-1 cells, as assessed with electrophysiological (whole-cell patch clamp) and microfluorimetry methods, showed that non-selective cationic currents were activated by allyl isothiocyanate (AITC) in Panc-1 cells and inhibited by the selective TRPA1 antagonist A-967079. The current elicited by the specific agonist was associated with a robust increase in intracellular Ca Topics: Adenocarcinoma; Calcium; Carcinogenesis; Carcinoma, Pancreatic Ductal; Cell Line, Tumor; Cell Movement; Electrophysiological Phenomena; Gene Expression Regulation, Neoplastic; Humans; Membrane Potentials; Oximes; Patch-Clamp Techniques; Transient Receptor Potential Channels; TRPA1 Cation Channel | 2021 |