a-317491 and Chronic-Pain

This study aimed to evaluate whether the development and/or maintenance of chronic-latent muscle hyperalgesia is modulated by P2X3 receptors. We also evaluate the expression of P2X3 receptors and PKCε of dorsal root ganglions during these processes. A mouse model of chronic-latent muscle hyperalgesia, induced by carrageenan and evidenced by PGE

Topics: Acute Pain; Animals; Chronic Pain; Disease Progression; Ganglia, Spinal; Male; Mice; Muscle, Skeletal; Myalgia; Pain Threshold; Phenols; Polycyclic Compounds; Purinergic P2X Receptor Antagonists; Receptors, Purinergic P2X3

We have recently demonstrated that pathological changes leading to increased bone resorption by osteoclast activation are related to the induction of pain-like behavior in ovariectomized (OVX) mice. In addition, bisphosphonate and the antagonist of transient receptor potential vanilloid type 1 (TRPV1), an acid-sensing nociceptor, improved the threshold value of pain-like behaviors accompanying an improvement in the acidic environment in the bone tissue based on osteoclast inactivation. The aim of this study was to evaluate the effect of (i) an inhibitor of vacuolar H(+) -ATPase, known as an proton pump, (ii) an antagonist of acid-sensing ion channel (ASIC) 3, as another acid-sensing nociceptor, and (iii) the P2X2/3 receptor, as an ATP-ligand nociceptor, on pain-like behavior in OVX mice. This inhibitor and antagonists were found to improve the threshold value of pain-like behavior in OVX mice. These results indicated that the skeletal pain accompanying osteoporosis is possibly associated with the acidic microenvironment and increased ATP level caused by osteoclast activation under a high bone turnover state.

Topics: Acid Sensing Ion Channels; Anilides; Animals; Bone and Bones; Bone Remodeling; Carbazoles; Chronic Pain; Cinnamates; Cnidarian Venoms; Cytokines; Female; Macrolides; Mice; Nociceptive Pain; Osteoporosis; Ovariectomy; Phenols; Polycyclic Compounds; Receptors, Purinergic P2X