Harringtonine and Necrosis

Harringtonine has been researched along with Necrosis* in 1 studies

Other Studies

1 other study(ies) available for Harringtonine and Necrosis

Article	Year
Induction of apoptosis by enediyne antitumor antibiotic C1027 in HL-60 human promyelocytic leukemia cells. Biochemical and biophysical research communications, 1995, Mar-08, Volume: 208, Issue:1 C1027, a new macromolecular antitumor antibiotic with an enediyne chromophore, displays extremely potent cytotoxicity against cancer cells. HL-60 human promyelocytic leukemia cells treated with C1027 (0.1-10 nM) for 2 hours resulted in morphological changes, including Hoechst 33342-stained condensed nuclei, condensation of nuclear chromatin, and nuclear fragmentation. Agarose gel electrophoresis of C1027-treated HL-60 cells showed a typical ladder-like pattern of DNA fragments. In addition, the apoptotic DNA peak of propidium iodide-stained nuclei was revealed by flow cytometry. Treatment of HL-60 cells with C1027 (5 nM) induced apoptosis in up to 79% of the cells. The results suggest that C1027 may exert antitumor activity by triggering apoptosis. Topics: Aminoglycosides; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Antineoplastic Agents; Apoptosis; Cell Line; Cell Survival; DNA Damage; DNA, Neoplasm; Dose-Response Relationship, Drug; Enediynes; Flow Cytometry; Harringtonines; Humans; Leukemia, Promyelocytic, Acute; Necrosis; Peptides; Tumor Cells, Cultured	1995

Article

Year

Induction of apoptosis by enediyne antitumor antibiotic C1027 in HL-60 human promyelocytic leukemia cells.

Biochemical and biophysical research communications, 1995, Mar-08, Volume: 208, Issue:1

C1027, a new macromolecular antitumor antibiotic with an enediyne chromophore, displays extremely potent cytotoxicity against cancer cells. HL-60 human promyelocytic leukemia cells treated with C1027 (0.1-10 nM) for 2 hours resulted in morphological changes, including Hoechst 33342-stained condensed nuclei, condensation of nuclear chromatin, and nuclear fragmentation. Agarose gel electrophoresis of C1027-treated HL-60 cells showed a typical ladder-like pattern of DNA fragments. In addition, the apoptotic DNA peak of propidium iodide-stained nuclei was revealed by flow cytometry. Treatment of HL-60 cells with C1027 (5 nM) induced apoptosis in up to 79% of the cells. The results suggest that C1027 may exert antitumor activity by triggering apoptosis.

Topics: Aminoglycosides; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Antineoplastic Agents; Apoptosis; Cell Line; Cell Survival; DNA Damage; DNA, Neoplasm; Dose-Response Relationship, Drug; Enediynes; Flow Cytometry; Harringtonines; Humans; Leukemia, Promyelocytic, Acute; Necrosis; Peptides; Tumor Cells, Cultured

1995