9r-(9alpha(z)-10alpha)-of-3--angeloyloxy-4--acetoxy-3--4--dihydroseselin and Myocardial-Ischemia

9r-(9alpha(z)-10alpha)-of-3--angeloyloxy-4--acetoxy-3--4--dihydroseselin has been researched along with Myocardial-Ischemia* in 1 studies

Other Studies

1 other study(ies) available for 9r-(9alpha(z)-10alpha)-of-3--angeloyloxy-4--acetoxy-3--4--dihydroseselin and Myocardial-Ischemia

Article	Year
Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes. Chinese medical journal, 2007, Dec-20, Volume: 120, Issue:24 Intermediate filament (IF) proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape. dl-praeruptorin A (Pd-Ia), a novel Ca2+-influx blocker and K+-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-Ia effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-Ia on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action.. Rats underwent a 30 minutes coronary occlusion followed by 120 minutes reperfusion. Assessment of desmin and vimentin content of myocardiocytes was performed by immunohistochemistry, Western blot, Hematoxylin-Eosin staining and densitometry.. Pretreatment with i.v. infusion of Pd-Ia prior to ischemia significantly increased desmin and vimentin content and alleviated defects caused by the ischemia/reperfusion insult, e.g. with Pd-Ia at a dose of 0.5 or 1.0 mg/kg, integrated density values of desmin were increased from 61 478 +/- 10 074 to 177 408 +/- 10 395 and 195 784 +/- 20 057, and vimentin from 59 189 +/- 19 853 to 164 781 +/- 19 543 and 185 696 +/- 20 957 (P < 0.01, vs placebo), respectively. The recovery of desmin seemed to be stronger and appeared earlier than that of vimentin.. Pd-Ia protectively increased IF desmin and vimentin content in ischemia/reperfusion myocardiocytes, which might be partially responsible for its cardioprotection against ischemia. Topics: Animals; Blotting, Western; Calcium; Coumarins; Desmin; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Female; Immunohistochemistry; Male; Myocardial Ischemia; Myocardial Reperfusion Injury; Myocytes, Cardiac; Rats; Rats, Wistar; Vimentin	2007

Article

Year

Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes.

Chinese medical journal, 2007, Dec-20, Volume: 120, Issue:24

Intermediate filament (IF) proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape. dl-praeruptorin A (Pd-Ia), a novel Ca2+-influx blocker and K+-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-Ia effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-Ia on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action.. Rats underwent a 30 minutes coronary occlusion followed by 120 minutes reperfusion. Assessment of desmin and vimentin content of myocardiocytes was performed by immunohistochemistry, Western blot, Hematoxylin-Eosin staining and densitometry.. Pretreatment with i.v. infusion of Pd-Ia prior to ischemia significantly increased desmin and vimentin content and alleviated defects caused by the ischemia/reperfusion insult, e.g. with Pd-Ia at a dose of 0.5 or 1.0 mg/kg, integrated density values of desmin were increased from 61 478 +/- 10 074 to 177 408 +/- 10 395 and 195 784 +/- 20 057, and vimentin from 59 189 +/- 19 853 to 164 781 +/- 19 543 and 185 696 +/- 20 957 (P < 0.01, vs placebo), respectively. The recovery of desmin seemed to be stronger and appeared earlier than that of vimentin.. Pd-Ia protectively increased IF desmin and vimentin content in ischemia/reperfusion myocardiocytes, which might be partially responsible for its cardioprotection against ischemia.

Topics: Animals; Blotting, Western; Calcium; Coumarins; Desmin; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Female; Immunohistochemistry; Male; Myocardial Ischemia; Myocardial Reperfusion Injury; Myocytes, Cardiac; Rats; Rats, Wistar; Vimentin

2007