9-diazomethylanthracene and Asthma

Plasma prostaglandin (PG) and thromboxane (Tx) levels from bronchial asthmatic patients were assayed by 9-anthryldiazomethane reversed-phase high-performance liquid chromatography method. TxB2 levels from stable bronchial asthmatic patients were significantly higher than those from normal healthy subjects. PGF2 alpha levels were significantly elevated in atopic patients compared with nonatopic ones. PG patterns differed between asthmatic and nonasthmatic conditions in the same patients. PGF2 alpha levels were significantly elevated in the patients with spontaneous attacks compared with those without attacks and 6-keto PGF1 alpha levels were significantly lower in bronchial asthmatic patients having attacks than in the patients without attacks. There were no significant differences of PG levels between mild and moderate asthmatic subjects. Aminophylline injection in patients with mild spontaneous attacks had no significant effects on PG levels which were compared before and after injection.

Topics: Adult; Aminophylline; Anthracenes; Asthma; Chromatography, High Pressure Liquid; Female; Fluorescent Dyes; Humans; Male; Middle Aged; Prostaglandins; Thromboxane B2

We report here an efficient method for simultaneous and quantitative determinations of plasma prostaglandins (PGs) from asthmatic patients using 9-anthryldiazomethane-reversed-phase high performance liquid chromatography (HPLC). Detection and simultaneous quantitation of 20 pg PGE1, PGE2, PGF2 alpha and 100 pg 6-keto PGF1 alpha, thromboxane B2 (TXB2) was possible by the present method. Preliminary studies showed that plasma TXB2 levels from stable asthmatic patients were elevated compared with normal healthy subjects, suggesting pathophysiologic implications regarding PGs and TXB2 in asthma may be elicited by the present HPLC method.

Topics: Anthracenes; Asthma; Chromatography, High Pressure Liquid; Dinoprost; Dinoprostone; Fluorometry; Humans; Microchemistry; Prostaglandins; Radioimmunoassay; Thromboxane B2; Time Factors

To evaluate some of the pathologic roles of prostaglandins (PGs) in bronchial asthma, plasma PGs levels in bronchial asthmatic patients were measured by the 9-anthryldiazomethane-HPLC method. Plasma TXB2 levels from stable bronchial asthmatic patients were higher than those from healthy subjects. Plasma PGF2 alpha levels were more elevated in atopic patients than in non-atopic ones. Plasma PGF2 alpha levels were more elevated in patients who suffered from attacks than in those who did not and plasma 6-keto PGF1 alpha levels were lower in patients who suffered from attacks than in those who did not. There was no significant difference in plasma TXB2 and PGs levels between mild and moderate asthmatic subjects. In the patients with mild attacks, aminophylline injection showed no significant effect on plasma TXB2 and PGs levels before and after the injection. These results suggest some of the roles of PGs in the pathogenesis of bronchial asthma.

Topics: Adult; Aged; Anthracenes; Asthma; Chromatography, High Pressure Liquid; Humans; Middle Aged; Prostaglandins