Compounds > 9-deoxy-9-10-didehydro-12-13-didehydro-13-14-dihydroprostaglandin-d2

9-deoxy-9-10-didehydro-12-13-didehydro-13-14-dihydroprostaglandin-d2 and Neuroblastoma

9-deoxy-9-10-didehydro-12-13-didehydro-13-14-dihydroprostaglandin-d2 has been researched along with Neuroblastoma* in 2 studies

Other Studies

2 other study(ies) available for 9-deoxy-9-10-didehydro-12-13-didehydro-13-14-dihydroprostaglandin-d2 and Neuroblastoma

Article	Year
Delta 12-prostaglandin J2 mimics heat shock in inducing cell cycle arrest at G1 phase. Biochemical and biophysical research communications, 1991, Sep-30, Volume: 179, Issue:3 Using a human neuroblastoma cell line GOTO, the effects of delta 12-prostaglandin (PG) J2 on the modulation of cell cycle progression and protein synthesis were examined in comparison with those caused by heat shock (HS). delta 12-PGJ2 induced G1 arrest, the peak of which was obtained at 24 h and continued for 72 h. HS was found to induce G1 arrest earlier than delta 12-PGJ2. Furthermore, sequential HS could maintain G1 arrest. delta 12-PGJ2 induced the synthesis of several heat shock proteins (HSPs) in a manner similar to HS. Using immunoblot analysis, HSP72 was detected prior to inducing G1 arrest and accumulated during the subsequent 72h. The content of HSP72 induced by HS also correlated well with the induction, release, and maintenance of G1 arrest. In addition, both delta 12-PGJ2 and HS induced HSP72 mRNA and simultaneously suppressed N-myc mRNA expression. These results suggest that delta 12-PGJ2 and HS regulate cell cycle progression of GOTO cells via similar mechanisms. Topics: Blotting, Northern; Cell Cycle; Cell Line; DNA, Neoplasm; Flow Cytometry; G1 Phase; Genes, myc; Heat-Shock Proteins; Hot Temperature; Humans; Kinetics; Neoplasm Proteins; Neuroblastoma; Prostaglandin D2; RNA, Messenger	1991
Cycloheximide reduces PGD2 or delta 12-PGJ2 cytotoxicity on NCG cells. Prostaglandins, 1986, Volume: 32, Issue:4 To study the precise mechanism of cytotoxic activity of PGD2 or delta 12-PGJ2 (a biologically active metabolite of PGD2), we examined the effect of various compounds on PGD2 or delta 12-PGJ2 cytotoxicity, using a human neuroblastoma cell line (NCG). Cycloheximide (CHM) specifically protected PGD2 cytotoxicity on NCG cells. When delta 12-PGJ2 was tested, CHM exhibited a similar rescue effect. Puromycin, mitomycin C, and alpha-amanitin did not affect PGD2 or delta 12-PGJ2 cytotoxicity. Emetine showed a variable and no consistent rescue effect CHM may have been active at the primary site where PGD2 or delta 12-PGJ2 exerts its cytotoxicity. This is the first report indicating that CHM reduces the cytotoxicity induced by PGD2 or delta 12-PGJ2. Topics: Amanitins; Cell Line; Cell Survival; Cycloheximide; Drug Interactions; Emetine; Humans; Mitomycin; Mitomycins; Neuroblastoma; Prostaglandin D2; Prostaglandins D; Puromycin	1986

Article

Year

Delta 12-prostaglandin J2 mimics heat shock in inducing cell cycle arrest at G1 phase.

Biochemical and biophysical research communications, 1991, Sep-30, Volume: 179, Issue:3

Using a human neuroblastoma cell line GOTO, the effects of delta 12-prostaglandin (PG) J2 on the modulation of cell cycle progression and protein synthesis were examined in comparison with those caused by heat shock (HS). delta 12-PGJ2 induced G1 arrest, the peak of which was obtained at 24 h and continued for 72 h. HS was found to induce G1 arrest earlier than delta 12-PGJ2. Furthermore, sequential HS could maintain G1 arrest. delta 12-PGJ2 induced the synthesis of several heat shock proteins (HSPs) in a manner similar to HS. Using immunoblot analysis, HSP72 was detected prior to inducing G1 arrest and accumulated during the subsequent 72h. The content of HSP72 induced by HS also correlated well with the induction, release, and maintenance of G1 arrest. In addition, both delta 12-PGJ2 and HS induced HSP72 mRNA and simultaneously suppressed N-myc mRNA expression. These results suggest that delta 12-PGJ2 and HS regulate cell cycle progression of GOTO cells via similar mechanisms.

Topics: Blotting, Northern; Cell Cycle; Cell Line; DNA, Neoplasm; Flow Cytometry; G1 Phase; Genes, myc; Heat-Shock Proteins; Hot Temperature; Humans; Kinetics; Neoplasm Proteins; Neuroblastoma; Prostaglandin D2; RNA, Messenger

1991

Cycloheximide reduces PGD2 or delta 12-PGJ2 cytotoxicity on NCG cells.

Prostaglandins, 1986, Volume: 32, Issue:4

To study the precise mechanism of cytotoxic activity of PGD2 or delta 12-PGJ2 (a biologically active metabolite of PGD2), we examined the effect of various compounds on PGD2 or delta 12-PGJ2 cytotoxicity, using a human neuroblastoma cell line (NCG). Cycloheximide (CHM) specifically protected PGD2 cytotoxicity on NCG cells. When delta 12-PGJ2 was tested, CHM exhibited a similar rescue effect. Puromycin, mitomycin C, and alpha-amanitin did not affect PGD2 or delta 12-PGJ2 cytotoxicity. Emetine showed a variable and no consistent rescue effect CHM may have been active at the primary site where PGD2 or delta 12-PGJ2 exerts its cytotoxicity. This is the first report indicating that CHM reduces the cytotoxicity induced by PGD2 or delta 12-PGJ2.

Topics: Amanitins; Cell Line; Cell Survival; Cycloheximide; Drug Interactions; Emetine; Humans; Mitomycin; Mitomycins; Neuroblastoma; Prostaglandin D2; Prostaglandins D; Puromycin

1986