8-oxo-7-8-dihydrodeoxyguanine and Urinary-Bladder-Neoplasms

Oxidative stress and reactivation of long interspersed element-1 (LINE-1) are coincidently observed in bladder cancer (BlCa), but the mechanistic connection between these two oncogenic phenomena is unknown. Previously, we reported increases in oxidative stress and LINE-1 protein (ORF1p) expression in human BlCa tissues. In this study, we measured 5-methylcytosine (5mC), 8-hydroxydeoxyguanosine (8-OHdG), 8-oxoguanosine DNA glycosylase-1 (OGG1), H3K9me3 and HP1α in bladder tissues obtained from BlCa patients. Reactivation of LINE-1 by reactive oxygen species (ROS) through chromatin remodeling was investigated in seven BlCa cell lines. We found that 5mC was decreased, but 8-OHdG, H3K9me3 and HP1α levels were increased in BlCa tissues relative to the adjacent non-cancerous tissues. OGG1, H3K9me3 and HP1α expression in BlCa tissues were positively correlated with 8-OHdG levels. Following H

Topics: 8-Hydroxy-2'-Deoxyguanosine; Aged; Case-Control Studies; Chromatin; Chromobox Protein Homolog 5; DNA Glycosylases; DNA Methylation; Epigenesis, Genetic; Female; Humans; Long Interspersed Nucleotide Elements; Male; Oxidative Stress; Promoter Regions, Genetic; Reactive Oxygen Species; Tumor Cells, Cultured; Urinary Bladder Neoplasms