8-oxo-2--deoxyadenosine and Stroke

Enhanced oxidative stress occurs in spontaneously hypertensive stroke-prone rats (SHRSP), and is important in blood-brain barrier (BBB) disruption. Hydrogen can exert potent protective cellular effects via reduction in oxidative stress in various diseases. The present study investigated whether long-term hydrogen treatment can improve neurological function outcome in the SHRSP model, and the effects of hydrogen on BBB function, especially the oxidative stress and the activity of matrix metalloproteinases (MMPs) in this model. Fifty-six animals were randomly assigned to 2 groups and treated as follows: SHRSP treated with hydrogen-rich water (HRW) (HRW group, n = 28); and SHRSP treated with regular water (control group, n = 28). The effect of HRW on overall survival and neurological function, and the effects of HRW on reactive oxygen species, BBB function, and MMP activities were examined.. HRW treatment improved neurological function and tended to improve overall survival but without significant difference. The numbers of bleeds and infarcts were lower in the cortex and hippocampus in the HRW group. The HRW group exhibited a significantly lower number of 8-hydroxy-2'-deoxyguanosine-positive cells and vessels of extravasated albumin in the hippocampus compared with the control group. MMP-9 activity was reduced in the hippocampus in the HRW group compared with the control group.. The present study suggests that ingestion of HRW can improve neurological function outcome in the SHRSP model. This beneficial effect may be due to attenuation of BBB disruption via reduction in reactive oxygen species and suppression of MMP-9 activity in the hippocampus.

Topics: Administration, Oral; Albumins; Animals; Blood Pressure; Blood-Brain Barrier; Body Weight; Cerebral Cortex; Deoxyadenosines; Drinking Water; Hippocampus; Hydrogen; Hypertension; Male; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Neuroprotective Agents; Oxidative Stress; Random Allocation; Rats, Inbred SHR; Stroke

Our aim was to explore whether free radical damage against DNA exists or not, in human stroke patients.. Urinary 8-hyroxy-2'-deoxyguanosine (8-OHdG) and serum S100beta were measured in acute cardioembolic stroke patients with relatively severe symptoms. During days 3--5 after the onset of stroke, urine was collected for 24 hours for 8-OHdG measurement and serum was sampled for S100beta analysis every day.. The total urinary 8-OHdG content was significantly higher in stroke patients than in non-stroke inpatients (p<0.01). The average value was more than twice the value of that of non-stroke inpatients. The total urinary 8-OHdG contents showed significant correlation (r=0.87, p<0.01) with serum S100beta values, which are reportedly related to brain damage.. These findings suggest that urinary 8-OHdG in acute stroke patients may originate from brain tissue, and DNA damage also exists in the brain of stroke patients.

Topics: Aged; Carotid Artery Thrombosis; Deoxyadenosines; Female; Humans; Magnetic Resonance Imaging; Male; Nerve Growth Factors; S100 Calcium Binding Protein beta Subunit; S100 Proteins; Statistics as Topic; Stroke