8-nitroguanosine and Cardiomegaly

8-nitroguanosine has been researched along with Cardiomegaly* in 1 studies

Other Studies

1 other study(ies) available for 8-nitroguanosine and Cardiomegaly

ArticleYear
Important Role of Endothelial Caveolin-1 in the Protective Role of Endothelium-dependent Hyperpolarization Against Nitric Oxide-Mediated Nitrative Stress in Microcirculation in Mice.
    Journal of cardiovascular pharmacology, 2018, Volume: 71, Issue:2

    Nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) play important roles in maintaining cardiovascular homeostasis. We have previously demonstrated that endothelial NO synthase (eNOS) plays diverse roles depending on vessel size, as a NO generating system in conduit arteries and an EDH-mediated system in resistance arteries, for which caveolin-1 (Cav-1) is involved. However, the physiological role of endothelial Cav-1 in microvessels remains to be elucidated.. We newly generated endothelium-specific Cav-1-knockout (eCav-1-KO) mice. eCav-1-KO mice showed loss of endothelial Cav-1/eNOS complex and had cardiac hypertrophy despite normal blood pressure. In eCav-1-KO mice, as compared to wild-type controls, the extent of eNOS phosphorylation at inhibitory Thr495 was significantly reduced in mesenteric arteries and the heart. Isometric tension and Langendorff-perfused heart experiments showed that NO-mediated responses were enhanced, whereas EDH-mediated responses were reduced in coronary microcirculation in eCav-1-KO mice. Immunohistochemistry showed increased level of 8-nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP), a marker of nitrative stress, in the heart from eCav-1-KO mice. S-guanylation of cardiac H-Ras in eCav-1-KO mice was also significantly increased compared with wild-type controls.. These results suggest that eCav-1 is involved in the protective role of EDH against nitrative stress caused by excessive NO to maintain cardiac microvascular homeostasis.

    Topics: Animals; Biological Factors; Cardiomegaly; Caveolin 1; Coronary Vessels; Cyclic GMP; Endothelial Cells; Guanosine; Isolated Heart Preparation; Male; Mesenteric Arteries; Mice, Inbred C57BL; Mice, Knockout; Microvessels; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase Type III; Nitro Compounds; Nitrosative Stress; Phosphorylation; Protein Processing, Post-Translational; Proto-Oncogene Proteins p21(ras); Signal Transduction; Vasodilator Agents

2018