8-hydroxyguanine and Uterine-Cervical-Neoplasms

8-hydroxyguanine has been researched along with Uterine-Cervical-Neoplasms* in 2 studies

Other Studies

2 other study(ies) available for 8-hydroxyguanine and Uterine-Cervical-Neoplasms

Article	Year
Lowered nudix type 5 (NUDT5) expression leads to cell cycle retardation in HeLa cells. Molecular and cellular biochemistry, 2012, Volume: 363, Issue:1-2 The molecule 8-oxo-7,8-dihydroguanine (8-oxoGua), an oxidized form of guanine, can pair with adenine or cytosine during nucleic acid synthesis. Moreover, RNA containing 8-oxoGua causes translational errors, thus leading to the production of abnormal proteins. Human NUDT5, a MutT-related protein, catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. To investigate the biological roles of NUDT5 in mammalian cells, we established cell lines with decreased level of NUDT5 expression. In NUDT5 inhibited cells, the RNA oxidation was not significantly higher than that of normal cells. However, the cell cycle G1 phase was significantly delayed, and cell numbers in both S and G2/M phases were reduced, indicating that cell proliferation was hampered by NUDT5 suppression. Key proteins for preventing the G1-S transition, including p53, p16, and Rb were increased, while the Rb phosphorylation was decreased. These results suggested that the NUDT5 protein may play significant roles in regulating the G1-S transition in mammalian cells. Topics: Apoptosis; Cell Proliferation; Cell Survival; Down-Regulation; Female; G1 Phase Cell Cycle Checkpoints; Gene Expression Regulation, Neoplastic; Guanine; HeLa Cells; Humans; Hydrolysis; Oxidation-Reduction; Pyrophosphatases; RNA Interference; RNA, Neoplasm; Uterine Cervical Neoplasms	2012
Oxidative DNA base damage in cancerous tissues of patients undergoing brachytherapy. Cancer letters, 1998, Oct-23, Volume: 132, Issue:1-2 This aim of this study was to measure the typical free radical-induced products of DNA bases in cellular DNA of cervical cancer tissues directly irradiated by applying brachytherapy to the patients. Significant increases in the amounts of modified bases over the control level were observed in the samples isolated after irradiation for all patients. These increases differed among patients and among products. The repair capacity and/or the amount of hypoxic cells inside the tumor may account for the different levels of modified bases. It is possible that the observed variabilities may account for the differences in clinical responses to brachytherapy. Topics: Adenine; Brachytherapy; Cytosine; DNA Damage; DNA, Neoplasm; Female; Gas Chromatography-Mass Spectrometry; Guanine; Humans; Oxidation-Reduction; Pyrimidines; Uracil; Uterine Cervical Neoplasms	1998

Article

Year

Lowered nudix type 5 (NUDT5) expression leads to cell cycle retardation in HeLa cells.

Molecular and cellular biochemistry, 2012, Volume: 363, Issue:1-2

The molecule 8-oxo-7,8-dihydroguanine (8-oxoGua), an oxidized form of guanine, can pair with adenine or cytosine during nucleic acid synthesis. Moreover, RNA containing 8-oxoGua causes translational errors, thus leading to the production of abnormal proteins. Human NUDT5, a MutT-related protein, catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. To investigate the biological roles of NUDT5 in mammalian cells, we established cell lines with decreased level of NUDT5 expression. In NUDT5 inhibited cells, the RNA oxidation was not significantly higher than that of normal cells. However, the cell cycle G1 phase was significantly delayed, and cell numbers in both S and G2/M phases were reduced, indicating that cell proliferation was hampered by NUDT5 suppression. Key proteins for preventing the G1-S transition, including p53, p16, and Rb were increased, while the Rb phosphorylation was decreased. These results suggested that the NUDT5 protein may play significant roles in regulating the G1-S transition in mammalian cells.

Topics: Apoptosis; Cell Proliferation; Cell Survival; Down-Regulation; Female; G1 Phase Cell Cycle Checkpoints; Gene Expression Regulation, Neoplastic; Guanine; HeLa Cells; Humans; Hydrolysis; Oxidation-Reduction; Pyrophosphatases; RNA Interference; RNA, Neoplasm; Uterine Cervical Neoplasms

2012

Oxidative DNA base damage in cancerous tissues of patients undergoing brachytherapy.

Cancer letters, 1998, Oct-23, Volume: 132, Issue:1-2

This aim of this study was to measure the typical free radical-induced products of DNA bases in cellular DNA of cervical cancer tissues directly irradiated by applying brachytherapy to the patients. Significant increases in the amounts of modified bases over the control level were observed in the samples isolated after irradiation for all patients. These increases differed among patients and among products. The repair capacity and/or the amount of hypoxic cells inside the tumor may account for the different levels of modified bases. It is possible that the observed variabilities may account for the differences in clinical responses to brachytherapy.

Topics: Adenine; Brachytherapy; Cytosine; DNA Damage; DNA, Neoplasm; Female; Gas Chromatography-Mass Spectrometry; Guanine; Humans; Oxidation-Reduction; Pyrimidines; Uracil; Uterine Cervical Neoplasms

1998