8-hydroxy-2--deoxyguanosine and Sarcopenia

Physical frailty is an aging-related clinical syndrome involving decreases in body weight, mobility, activity, and walking speed that occurs in individuals with sarcopenia and is accelerated by increased oxidative stress. Ninjin'yoeito, a traditional Japanese Kampo medicine, is used for treating conditions, including anemia and physical weakness. Here, we investigated whether ninjin'yoeito could improve physical frailty by controlling oxidative stress in the senescence-accelerated mouse prone 8 (SAMP8) model. First, SAMP8 mice were divided into two groups, ninjin'yoeito treated and untreated, with the former consuming a diet containing 3% ninjin'yoeito from 3 months of age. At 7 months of age, body weight, motor function, locomotor activity, and mean walking speed were measured. Subsequently, mice were euthanized and measured for muscle weight, 8-hydroxy-2'-deoxyguanosine levels in muscle and brain, and cleaved caspase-3 expression in brain. The results showed reductions in weight, locomotor function, locomotion, and average walking speed in the untreated group, which were significantly improved by ninjin'yoeito. Furthermore, 8-hydroxy-2'-deoxyguanosine levels were reduced in muscle and brain from ninjin'yoeito-treated mice, compared with the levels in untreated mice; cleaved caspase-3 expression was similarly reduced in brain from the treated mice, indicating reduced apoptosis. Our findings suggest that ninjin'yoeito inhibits sarcopenia-based physical frailty through its antioxidant effects.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Animals; Antioxidants; Body Weight; Caspase 3; Disease Models, Animal; Drugs, Chinese Herbal; Frailty; Mice; Sarcopenia

The accelerate loss of skeletal muscle mass, strength, and function, named sarcopenia, is a progressive and generalised skeletal muscle disorder, and it is always associated with increased outcomes including falls, frailty, and disability. Diabetes mellitus is associated with significant muscle and physical complications. We aimed at clarifying the changes and risk factors of skeletal muscle mass and strength in elderly with type 2 diabetes.. The study consisted of patients with type 2 diabetes (. T2DM patients exhibited lower muscle strength compared with the non-T2DM subjects (. These findings suggest that diabetic patients may be more susceptible to sarcopenia at an older age. And it also provides evidences that among elderly with diabetes mellitus, oxidative damage and HCY as well as IGF-1 are important predictors of age-dependent sarcopenia.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Age Factors; Aged; Biomarkers; Body Composition; Case-Control Studies; China; Cross-Sectional Studies; Diabetes Mellitus, Type 2; Female; Hand Strength; Homocysteine; Humans; Insulin-Like Growth Factor I; Male; Middle Aged; Muscle, Skeletal; Oxidative Stress; Risk Assessment; Risk Factors; Sarcopenia