8-hydroxy-2--deoxyguanosine and Polycystic-Ovary-Syndrome

Oxidative stress in the follicular fluid (FF) is thought to be responsible for the abnormal development of oocytes. In our study patients with polycystic ovarian syndrome (PCOS), endometriosis, and tubal infertility factor (TIF), and healthy women with a male factor of infertility, were prospectively enrolled. From each patient, a sample of individual FF was collected from a dominant follicle. Concentration levels of TAS, 8-IP, 8-OHdG, and AMH were determined. In women with PCOS, we found significantly lower values of oxidative stress markers in the FF. 8-IP and TAS levels were lower in the FF of women with endometriosis. In women with TIF, we also found significantly lower values of all tested markers in the FF, except for 8-OHdG and AMH. We wanted to see whether the biomarker measured in the FF in an individual diagnosis could predict a successfully obtained embryo from this particular follicle. The FF 8-OHdG result in PCOS patients stood out and proved to be a good predictive marker of matured and fertilized oocytes in these patients. Further research is needed to be able to apply the acquired knowledge in improving the outcome of IVF procedures.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Anti-Mullerian Hormone; Biomarkers; Dinoprost; Endometriosis; Female; Follicular Fluid; Humans; Infertility, Female; Live Birth; Oxidative Stress; Polycystic Ovary Syndrome; Pregnancy; Statistics, Nonparametric

Are daily cycles in urinary melatonin and oxidative stress marker levels (8-hydroxydeoxyguanosine) altered in PCOS, and is this associated with changes in sleep quality?. There is an association between elevated nighttime melatonin and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels, and poor sleep quality in our PCOS study group.. Women with PCOS are known to have poorer sleep. However, there have been few studies examining the possible association between melatonin levels and sleep quality in women with polycystic ovarian syndrome (PCOS).. This is a case-control study of PCOS (n = 26) and non-PCOS control (n = 26) subjects recruited from a tertiary gynaecological centre.. The participants were requested to complete sleep questionnaires for a month. In a subgroup from these cohorts (PCOS, n = 15; controls, n = 18), urine samples were also collected at various time points over a 24-h period. In addition, their sleep patterns and lighting environment were monitored for 3 consecutive days and nights using a wrist-mounted Actiwatch device.. PCOS women had significantly elevated night-time urinary levels of the melatonin metabolite 6-sulfatoxymelatonin (aMT6s) and of 8-OHdG (both at P < 0.05), as well as significantly reduced sleep quality (P < 0.05), compared with the controls.. Due to the small sample size of the study, further studies will be required to confirm our findings.. Our preliminary work provides a possible new insight into the interactions between melatonin, increased oxidative stress and sleep in women with PCOS.. The study was funded by the Faculty of Medicine, University of Southampton.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adolescent; Adult; Case-Control Studies; Deoxyguanosine; Female; Hormones; Humans; Melatonin; Monitoring, Physiologic; Oxidative Stress; Polycystic Ovary Syndrome; Sleep; Sleep Wake Disorders; Surveys and Questionnaires; Young Adult

Polycystic ovary syndrome (PCOS) has recently been linked with genomic instability and DNA damage. The aim of this study was to test genomic damage in women PCOS, using two different methods for assessing damage in both chromosome and base level. The study was performed on 36 newly diagnosed women with PCOS and 29 healthy women as controls. The micronucleus (MN) analysis used as a biomarker of chromosomal/DNA damage was performed in peripheral lymphocytes by cytokinesis-block method. 8-hydroxydeoxyguanosine (8-OHdG) levels used as a reliable marker of oxidative DNA damage were measured in plasma using an ELISA kit. We found that MN frequencies obtained from lymphocytes of the women with PCOS were significantly higher than those of controls (4.1 +/- 1.0 vs. 2.1 +/- 0.6, P = 0.001), whereas, no differences in 8-OHdG level were found between the patients with PCOS and controls (0.5 +/- 0.3 vs. 0.5 +/- 0.2, P = 0.858). These findings indicate that women with PCOS seem to have increased genomic instability, but do not appear to have oxidative DNA damage despite the increased oxidative stress associated with PCOS.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adolescent; Adult; Case-Control Studies; Chromosome Breakage; Deoxyguanosine; DNA Damage; Female; Humans; Micronucleus Tests; Oxidative Stress; Polycystic Ovary Syndrome; Young Adult

To investigate oxidative stress and angiogenetic factors in polycystic ovary syndrome (PCOS).. Prospective cohort study.. University outpatient clinic.. Fifty women with PCOS were divided into two groups: body mass index (BMI) >27 kg/m(2) (n = 25) and BMI <27 kg/m(2) (n = 25). The control group consisted of 20 age- and BMI-matched healthy women.. None.. Enzyme-linked immunosorbent assays were used to measure serum levels of 8-hydroxydeoxyguanosine (8-OHdG), angiopoietin-2, and vascular endothelial growth factor (VEGF).. Women with PCOS had significantly lower serum 8-OHdG levels (mean 137.8 pg/mL, range 53.4-282.9 pg/mL) compared with the control subjects (mean 219.78 pg/mL, 124.6-372.4 pg/mL). This difference was obvious in both lean (BMI <27 kg/m(2)) and obese (BMI >27 kg/m(2)) subjects. Concentrations of VEGF were higher among obese subjects with PCOS.. Serum 8-OHdG levels are significantly lower in women with PCOS than in healthy controls. The clinical significance of this finding is discussed.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Angiopoietin-2; Body Mass Index; Case-Control Studies; Deoxyguanosine; Down-Regulation; Female; Humans; Menstrual Cycle; Polycystic Ovary Syndrome; Vascular Endothelial Growth Factor A; Young Adult