8-hydroxy-2--deoxyguanosine and Periodontal-Pocket

The present study was carried out as a multicenter, randomized controlled, split-mouth clinical trial to evaluate the efficacy of locally delivered lycopene on periodontal health and gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG) in smokers and nonsmokers compared with periodontally healthy control subjects.. One hundred ten subjects including 50 smokers, 50 nonsmokers, and 10 controls participated in this study. Subjects in the smoker and nonsmoker groups had contralateral sites treated with lycopene gel and a placebo. Clinical parameters included recording site-specific measures of plaque, gingivitis, probing depth, and clinical attachment level. GCF 8-OHdG values were analyzed using a commercially available ELISA kit.. Compared with the placebo, lycopene-treated sites in smokers and nonsmokers showed significant reductions in probing depths and gain in the clinical attachment levels. However, there was no statistically significant difference in the clinical parameters when lycopene-treated sites in smokers and nonsmokers were compared, except for the reduction in the 8-OHdG levels. The 8-OHdG levels at 1 week and 3 months in sites treated with lycopene in the smoker and nonsmoker group were comparable with those in the periodontally healthy control group.. The gel formulation was effective in increasing clinical attachment and reducing gingival inflammation, probing depth, and oxidative injury compared with the placebo in smoking and nonsmoking subjects.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Administration, Topical; Adult; Antioxidants; Carotenoids; Chronic Periodontitis; Delayed-Action Preparations; Dental Plaque Index; Dental Scaling; Deoxyguanosine; Follow-Up Studies; Gels; Gingival Crevicular Fluid; Gingivitis; Humans; Lycopene; Male; Middle Aged; Oxidative Stress; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Placebos; Root Planing; Smoking; Treatment Outcome

Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long-term release of reactive oxygen species. A high number of reactive oxygen species result in periodontal tissue damage through multiple mechanisms such as lipid peroxidation, protein denaturation and DNA damage. The aim of this study was to evaluate DNA and oxidative damage in subjects with chronic or aggressive periodontitis and healthy controls.. Buccal mucosa cells and whole saliva were collected from 160 subjects, who were divided into three groups: subjects with chronic periodontitis (CP) (n = 58), subjects with aggressive periodontitis (AgP) (n = 42) and a control group (n = 60). DNA damage was determined by counting micronuclei (MN) and nuclear abnormalities (NAs) in exfoliated cells, including binucleated cells, cells with nuclear buds and karyolitic, karyorrhectic, condensed chromatin and pyknotic cells. The degree of oxidative stress was determined by quantifying 8-hydroxy-2'-deoxyguanosine (8-OHdG) in whole saliva.. Subjects with CP or AgP presented significantly more ( p < 0.05) MN and NAs and higher levels of 8-OHdG ( p < 0.05) compared with the control group.. Our results indicate that subjects with periodontitis (CP or AgP) exhibited an increase in the frequency of MN, NAs and 8-OHdG, which is directly related to DNA damage. In addition, a positive correlation exists between oxidative stress produced by periodontitis disease and MN.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Aggressive Periodontitis; Cell Nucleus; Chromatin; Chronic Periodontitis; Dental Plaque Index; Deoxyguanosine; DNA Damage; Female; Humans; Male; Micronuclei, Chromosome-Defective; Middle Aged; Mouth Mucosa; Oxidative Stress; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Saliva

The purpose of this study is to determine the serum levels of malondialdehyde (MDA), as a lipid peroxidation marker, and 8-hydroxydeoxyguanosine (8-OHdG), as an oxidative DNA damage marker, in patients with chronic periodontitis (CP) and hyperlipidemia.. A total of 74 individuals were divided into four age- and sex-matched groups: 18 patients with hyperlipidemia and CP (HLp), 18 periodontally healthy patients with hyperlipidemia (HLh), 19 systemically healthy individuals with CP (Cp), and 19 systemically and periodontally healthy controls (Ch). Clinical periodontal parameters were measured, and serum lipids, MDA, and 8-OHdG levels were assessed in blood samples.. 8-OHdG, MDA, probing depth, clinical attachment level, and percentage of sites bleeding on probing (BOP) were significantly higher in the HLp group than the Cp group. In the hyperlipidemic group, BOP was significantly correlated with total cholesterol, the ratio of total cholesterol to high-density lipoprotein cholesterol, and 8-OHdG levels. A significant correlation between 8-OHdG and MDA was also observed in the hyperlipidemia group.. In this study, serum MDA and 8-OHdG were found to be highest in the HLp group. The increased levels of MDA and 8-OHdG in HLp patients may be a result of a harmful oxidative status in association with hyperlipidemia and periodontitis.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Aged; Alveolar Bone Loss; Biomarkers; Case-Control Studies; Cholesterol; Cholesterol, HDL; Cholesterol, LDL; Cholesterol, VLDL; Chronic Periodontitis; Deoxyguanosine; DNA Damage; Female; Humans; Hyperlipidemias; Lipid Peroxidation; Male; Malondialdehyde; Middle Aged; Oxidative Stress; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Triglycerides

This study evaluates the effects of initial periodontal treatment on the gingival crevicular fluid (GCF) and salivary levels of 8-hydroxy-deoxyguanosine (8-OHdG) as a marker of oxidative deoxyribonucleic acid (DNA) damage in patients with chronic periodontitis (CP).. At baseline, clinical parameters were determined and GCF and saliva samples were obtained from 24 patients with CP and 24 individuals with clinically healthy periodontium. GCF, saliva samples, and clinical periodontal measurements were repeated at day 10, 1 month, and 3 months following initial periodontal therapy in patients with CP. 8-OHdG levels of GCF and saliva samples were investigated by using an enzyme-linked immunosorbent assay.. Statistically significant higher 8-OHdG levels of GCF and a significant decrease after initial periodontal therapy were determined in the CP group (P <0.001). A significant positive correlation was found between 8-OHdG levels of GCF and clinical periodontal measurements (P <0.001). However, salivary levels of 8-OHdG did not differ between groups or during initial periodontal therapy (P >0.05).. This study reveals that DNA injury and oxidative stress increase in tissue cells and especially in periodontal pockets in patients with CP, and the periodontal treatment results in a significant decrease of 8-OHdG levels in the GCF samples. To the best of our knowledge, this study evaluates for the first time, 8-OHdG levels in GCF, which is shown to be more useful as a biomarker than saliva. 8-OHdG was found to be important and may reveal the severity of periodontal disease and the effect of periodontal therapy.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adolescent; Adult; Biomarkers; Chronic Periodontitis; Dental Plaque; Dental Scaling; Deoxyguanosine; DNA Damage; Female; Follow-Up Studies; Gingival Crevicular Fluid; Humans; Male; Middle Aged; Oral Hygiene; Oxidative Stress; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Root Planing; Saliva; Young Adult

Microgravity is associated with an increase in peroxidative damage. The effect is more pronounced after long-duration space flights and can even last for several weeks after landing. The objective of the study is to determine the influence of simulated microgravity on the periodontal status and salivary and serum oxidant/antioxidant status of the body in simulated microgravity (-6° head-down-tilt [HDT) bed rest).. Twenty healthy male volunteers were studied before and after 60 days of simulated microgravity (-6° HDT bed rest). We measured salivary and serum oxidative markers, such as malondialdehyde (MDA), 8-oxo-7,8 dihydro-2 deoxyguanosine (8-OHdG), and vitamins C and E, and clinical periodontal parameters (probing depth [PD] and clinical attachment level [CAL]).. Serum and salivary vitamin C and E concentrations were significantly decreased, whereas MDA and 8-OHdG levels were significantly increased after 60 days of simulated microgravity. Serum and salivary markers showed a strong and significant correlation. CAL and PD were higher but not statistically significant in simulated microgravity.. This study suggests that oxidative stress in the microgravity environment was increased but did not significantly influence periodontal parameters after 2 months. Also, this study indicates the possibility that the findings may have a broader clinical relevance to patients on bed rest or who are physically inactive. Studies on larger patient samples and follow-up for a longer time are required to verify the relationship between antioxidant status in the space microgravity condition and periodontal health.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adolescent; Antioxidants; Ascorbic Acid; Dental Stress Analysis; Deoxyguanosine; Head-Down Tilt; Health Status; Humans; Male; Malondialdehyde; Oxidants; Oxidative Stress; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Prospective Studies; Saliva; Vitamin E; Weightlessness Simulation; Young Adult

There is an increasing body of evidence implicating reactive oxygen species in the pathogenesis of periodontal tissue destruction. 8-Hydroxy-deoxyguanosine (8-OHdG) is one of the most commonly used markers to evaluate oxidative damage in a number of disorders including chronic inflammatory diseases. The aim of the present study was to evaluate 8-OHdG levels in whole saliva of patients with periodontitis and to assess the changes after initial treatment.. Saliva samples were collected from 78 patients with untreated periodontitis and 17 healthy control subjects. Clinical parameters and levels of 8-OHdG were assessed first to establish a baseline and again after initial periodontal treatment from 15 patients. 8-OHdG levels were determined by enzyme-linked immunosorbent assay.. The mean value of 8-OHdG in the saliva of periodontally diseased subjects, 4.28 +/- 0.10 ng/ml, was significantly higher (P<0.01) than that of clinically healthy subjects (1.56 +/- 0.10 ng/ml). A significant decrease in salivary 8-OHdG was observed after therapy (P<0.01).. In the present study, we evaluated for the first time 8-OHdG levels in whole saliva of patients with periodontitis and assessed changes after initial periodontal treatment. Our study indicated that 8-OHdG levels in saliva appear to reflect the status of periodontal health.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Aged; Biomarkers; Chronic Disease; Dental Scaling; Deoxyguanosine; DNA Damage; Female; Follow-Up Studies; Gingival Hemorrhage; Humans; Male; Middle Aged; Oral Hygiene; Oxidative Stress; Periodontal Attachment Loss; Periodontal Pocket; Periodontitis; Reactive Oxygen Species; Root Planing; Saliva; Smoking; Statistics as Topic; Statistics, Nonparametric