8-hydroxy-2--deoxyguanosine and Osteoarthritis

Plasma homocysteine (tHcy) is a risk factor for cardio-vascular diseases. Furthermore it has been associated with antioxidative status. Additionally balneotherapeutic sulphur baths have been shown to influence antioxidative status.. 40 patients with degenerative osteoarthrosis were randomised into two equal groups, a treatment group, receiving stationary spa therapy plus daily sulphur baths (sulphur group) and a control group receiving spa therapy alone (control group). Blood tHcy levels and urinary 8-OHdG (an indicator for oxidative stress) were measured at the beginning and the end of spa therapy.. tHcy (micromol/l) was significantly reduced from 11.41 (+/-2.91) to 10.55 (+/-2.28) in the sulphur group (p=0.016) and rose insignificantly from 12.93 (+/-2.28) to 13.80 (+/-3.87) in the control group. 8-OHdG (ng 8-OHdG/mg creatinine) declined from 18.00 (+/-18.28) to 11.16 (+/-5.33) in the sulphur group (n.s.) and from 17.91 (+/-5.87) to 18.17 (+/-5.70) in the control group (n.s.). Differences between the two groups showed significant effects of sulphur baths for tHcy (p=0.006) but not for 8-OHdG (p=0.106).. Sulphur baths exert beneficial effects on plasma tHcyt whereas effects on 8-OHdG seem to be unlikely.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Antioxidants; Baths; Creatine; Deoxyguanosine; Female; Homocysteine; Humans; Male; Middle Aged; Osteoarthritis; Sulfur

In order to study molecular mechanism of osteoarthrosis, we used molecular techniques to detect DNA oxidative damage of cartilage. There were 10 samples from clinical operation including 5 cases of replacement of hip joint, 4 degenerative cartilage of knee osteoarthrosis, and 1 free body of knee joint. DNA from all samples was run out on an agarose gel and appeared classic nucleosomal ladders. (8-hydroxyl-2'-deoxyguanosine)/ml (8-OHdG) in DNA samples was measured by high-performance liquid chromatography with electrochemical detection (HPLC-EC). The results showed that the levels of 8-OHdG were increased to 0.11-0.26 ng.ml-1, average 0.177 ng.ml-1. Normal controls were lower than 0.05 ng.ml-1. The high 8-OHdG in DNA led to a misreading of affected templates and base-pair exchange, which could be a sensitive indicator about oxidative DNA damage, which was produced by free radicals or other DNA damage agents. The result suggests that oxidative DNA damage may be the molecular mechanism of osteoarthrosis.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Aged; Apoptosis; Biomarkers; Cartilage, Articular; Deoxyguanosine; DNA Damage; Female; Humans; Male; Middle Aged; Osteoarthritis