8-hydroxy-2--deoxyguanosine and Eczema

The significance of oxidative stress in pathogenesis of childhood asthma was recognized, but its role in the clinical manifestations of disease is still unclear.. The study was conducted in 96 asthmatic children. The urinary biomarker of oxidative stress, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG/creatinine) was determined by using HPLC-MS/MS. ELISA was performed to measure myeloperoxidase (MPO) and Cu,Zn- superoxide dismutase (Cu,Zn-SOD) in serum.. Logistic regression analysis revealed that female gender, tobacco smoke exposure, and increased 8-oxodG/creatinine were associated with risk for intermittent asthma, while the positive allergy test and increased Cu,Zn-SOD were associated with eczema in asthmatic children. Higher MPO (p = 0.033), and percent of granulocytes (p = 0.030) were found in severe persistent asthma in comparison to intermittent or mild persistent asthma.. The main findings that TSE-induced oxidative stress is a risk for intermittent asthma and eczema may be clinically significant for the disease prevention and therapeutic improvements.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adolescent; Asthma; Biomarkers; Child; Child, Preschool; Deoxyguanosine; Eczema; Female; Humans; Male; Oxidative Stress; Peroxidase; Superoxide Dismutase-1; Tobacco Smoke Pollution; Young Adult

Ambient fine and ultrafine particles (UFPs) in urban air are known to contribute to inflammatory and allergic disease. It has been suggested that oxidative stress is an underlying mechanism for the detrimental health effects. The objective of this study was to investigate the short-term effect of ambient UFPs and particulate polycyclic aromatic hydrocarbons (PAHs) on urinary 8-hydroxyl-2-deoxyguanosine (8-OHdG) concentrations in children with and without eczema. Spot urine samples were collected from 84 children twice weekly for 61 days and 8-OHdG content was measured. Significant associations were found between the ambient UFPs and particle bound PAHs and increase in urinary 8-OHdG levels. An inter-quartile range (IQR) increase in the UFP concentration in the 24-h (IQR, 32,300/m(3)) period preceding urine collection was significantly associated with a 5.7% (95% confidence interval [CI], 0.16-1.27%) increase in the urinary 8-OHdG level children with AD. In children without eczema, such short-term effect of previous day UFPs on urinary 8-OHdG was not observed. There were no significant positive associations between the mass fraction of PMs and urinary 8-OHdG. The results suggest that short-term exposure to ambient UFPs plays a critical role in PM induced oxidative stress in children with eczema.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Child; Creatinine; Deoxyguanosine; Eczema; Environmental Exposure; Enzyme-Linked Immunosorbent Assay; Humans; Oxidative Stress; Particle Size; Particulate Matter; Polycyclic Aromatic Hydrocarbons; Republic of Korea