8-hydroxy-2--deoxyguanosine and Cadmium-Poisoning

The aim of this study was to investigate if purple carrot extract is able to protect against the noxious activities induced by cadmium exposure in multiple organs of rats. For this purpose, histopathological analysis, genotoxicity and oxidative status were investigated in this setting. A total of twenty Wistar rats weighing 250g on the average, and 8 weeks age were distributed into four groups (n=5), as follows: Control group (non-treated group, CTRL); Cadmium group (Cd) and Purple carrot extract groups at 400mg/L or 800mg/L. Histopathological analysis revealed that liver from animals treated with purple carrot extract improved tissue degeneration induced by cadmium intoxication. Genetic damage was reduced in blood and hepatocytes as depicted by comet and micronucleus assays in animals treated with purple carrot extract. SOD-CuZn and cytocrome C gene expression increased in groups treated with purple carrot extract. Purple carrot extract also reduced the 8OHdG levels in liver cells when compared to cadmium group. Taken together, our results demonstrate that purple carrot extract is able to protect against cadmium intoxication by means of reducing tissue regeneration, genotoxicity and oxidative stress in multiple organs of Wistar rats.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Animals; Bone Marrow Cells; Cadmium Poisoning; Comet Assay; Cytochromes c; Daucus carota; Deoxyguanosine; DNA Damage; Gene Expression Regulation; Immunohistochemistry; Liver; Micronucleus Tests; Mutagens; Organ Specificity; Oxidative Stress; Plant Extracts; Rats, Wistar; Real-Time Polymerase Chain Reaction; Spectrometry, Mass, Electrospray Ionization; Weight Gain