8-azidoethidium and Parasitemia

Trypanosoma brucei E164 and a dyskinetoplastic derivative, Dysk164, were injected into mice that were treated subsequently with methylglyoxal-bis-guanylhydrazone, berenil, ethidium bromide, and acriflavine. Additionally, parasites were photoaffinity labeled with ethidium monoazide to effect covalent drug attachment prior to injection into animals. In all cases, killing of animals with E164 was blocked by the drug treatment, whereas killing due to Dysk164 was not. These findings are consistent with the view that the intact kinetoplast plays an essential role in the action of these drugs.

Topics: Acriflavine; Animals; Azides; Diminazene; Dose-Response Relationship, Drug; Drug Resistance; Ethidium; Female; Light; Mice; Mitochondria; Mitoguazone; Mutation; Parasitemia; Trypanocidal Agents; Trypanosoma brucei brucei; Trypanosomiasis, African