7-methoxyisoflavone and Colonic-Neoplasms

Isoflavones are a class of polyphenols that contain various substituents such as hydroxy, methoxy, and glycosyl groups. Methoxy groups are known to increase cell permeability and stability, but small structural changes can result in large differences in biological activity. In this study, the anticancer activities of several methoxy isoflavones were tested using a clonogenic survival assay. The relationship between structural properties of methoxy isoflavones and their anticancer activities on HCT116 colon cancer cell lines were studied quantitatively using comparative molecular field analysis and comparative molecular similarity indices analysis. The purpose of this study was to identify structural changes in isoflavones that increase the inhibitory effect on HCT116 colon cancer cell clonogenicity.

Topics: Antineoplastic Agents; Biological Assay; Cell Line, Tumor; Cell Proliferation; Cell Survival; Clone Cells; Colonic Neoplasms; Flavonoids; HCT116 Cells; Humans; Isoflavones; Models, Molecular; Molecular Structure

It has been previously shown that some flavonoids inhibit NF-κB; however, the structure-activity relationships between chalcone, flavanone, flavone, and isoflavone derivatives and their TNFα induced NF-κB inhibitory effects on HCT116 human colon cancer cells have not yet been reported. Therefore, in this study, the effects of flavonoid structure on inhibition of NF-κB were investigated. Based on the combined results of this study, the structure of the flavonoids was shown to affect NF-κB activation.

Topics: Anti-Inflammatory Agents; Antineoplastic Agents; Cell Line, Tumor; Colonic Neoplasms; Flavonoids; Humans; Models, Molecular; NF-kappa B; Structure-Activity Relationship; Tumor Necrosis Factor-alpha