7-deazaguanine and Fragile-X-Syndrome

7-deazaguanine has been researched along with Fragile-X-Syndrome* in 1 studies

Other Studies

1 other study(ies) available for 7-deazaguanine and Fragile-X-Syndrome

Article	Year
A simple fragile X PCR assay with 7-deazaguanine-substituted DNA visualized by ethidium bromide. Molecular and cellular probes, 1994, Volume: 8, Issue:2 A deazaguanine-substituted DNA PCR product from FMR-1 (the fragile X mental retardation syndrome gene) can be efficiently visualized with ethidium bromide on standard agarose gels. Normal-sized alleles (less than 54 CGG repeats) generated strong, easily visible bands in the expected size range of 491-635 bp. Southern blot analysis and radioactive PCR on sequencing gels were used to verify that the 74 males (out of 245 total tested) whose DNA failed to generate a visible band contained premutations or full mutations. This technique can be used as an inexpensive screen for fragile X syndrome among developmentally delayed males. Topics: Alleles; Base Sequence; Blotting, Southern; DNA; Ethidium; Fragile X Syndrome; Guanine; Humans; Male; Molecular Sequence Data; Mutation; Polymerase Chain Reaction	1994

Article

Year

A simple fragile X PCR assay with 7-deazaguanine-substituted DNA visualized by ethidium bromide.

Molecular and cellular probes, 1994, Volume: 8, Issue:2

A deazaguanine-substituted DNA PCR product from FMR-1 (the fragile X mental retardation syndrome gene) can be efficiently visualized with ethidium bromide on standard agarose gels. Normal-sized alleles (less than 54 CGG repeats) generated strong, easily visible bands in the expected size range of 491-635 bp. Southern blot analysis and radioactive PCR on sequencing gels were used to verify that the 74 males (out of 245 total tested) whose DNA failed to generate a visible band contained premutations or full mutations. This technique can be used as an inexpensive screen for fragile X syndrome among developmentally delayed males.

Topics: Alleles; Base Sequence; Blotting, Southern; DNA; Ethidium; Fragile X Syndrome; Guanine; Humans; Male; Molecular Sequence Data; Mutation; Polymerase Chain Reaction

1994