7-3--dihydroxy-4--methoxyisoflavone and Diabetes--Gestational

Gestational diabetes mellitus (GDM) is a medical complication and metabolic disorder associated with pregnancy. Calycosin is a traditional Chinese herbal medicine that is used for the treatment of multiple diseases. This study focused on exploring the effects and underlying mechanisms of Calycosin on GDM.. The db/+ diabetic mice model of GDM was used to evaluate the effects of calycosin administration on the symptoms of GDM mice. Blood glucose, cytokine production (interleukin 6, IL-6; tumor necrosis factor-α, TNF-α), and insulin levels were measured by ELISA assay. The expression level of signal transducer and activator of transcription 3 (STAT3), ring finger protein 38 (RNF38), and SH2-containing protein tyrosine phosphatase 1 (SHP-1) were determined by Western Blot assay. Beta cell proliferation was assessed by CCK-8 assay.. Our data indicated that administration of calycosin significantly improved the GDM symptoms in pregnant db/+ mice as demonstrated by reduced blood glucose, TNF-a, and IL-6 levels as well as increased insulin level, and body weight. Furthermore, we revealed that RNF38/SHP-1/STAT3 signaling should play a critical role in calycosin-promoted beta cell function, and forced expression of RNF38 attenuated the positive effects of calycosin on beta cells.. Our study implied that calycosin exerts favorable effects on GDM mice

Topics: Animals; Carrier Proteins; Diabetes Mellitus, Experimental; Diabetes, Gestational; Dimethyl Sulfoxide; Dose-Response Relationship, Drug; Female; Gene Expression; Inflammation Mediators; Insulin-Secreting Cells; Isoflavones; Mice; Mice, Inbred C57BL; Mice, Transgenic; Pregnancy; Random Allocation