6-ketoprostaglandin-f1-alpha and Leiomyoma

In order to investigate the production of eicosanoids in human endometrium, myometrium, leiomyoma, adenomyosis, normal ovary, non-endometrial cyst and endometrial cyst, slices of each tissue were incubated. 6-Keto-prostaglandin (PG) F1 alpha, thromboxane (TX) B2, PGF2 alpha and PGE2 concentrations in the incubation medium were measured by direct RIA. 6-Keto-PGF1 alpha production of adenomyosis was significantly higher than that of endometrium, myometrium and leiomyoma, especially in the menstrual phase. The production of eicosanoids in endometrial cyst was significantly higher than that of non-endometrial cyst and normal ovary. These results suggest that endometriosis is associated with increased eicosanoid production in vivo.

Topics: 6-Ketoprostaglandin F1 alpha; Adult; Cysts; Dinoprost; Dinoprostone; Eicosanoids; Endometriosis; Female; Humans; Leiomyoma; Middle Aged; Myometrium; Ovary; Thromboxane B2; Uterine Neoplasms

To study the role of prostacyclin (PGI2) and thromboxane A2 (TxA2) in uterine tumors, pieces of endometrial cancer (n = 12) and leiomyomas (n = 12) were incubated in vitro, and the productions of 6-keto-prostaglandin F1a (6-keto-PGF1a, a hydration product of PGI2) and thromboxane B2 (TxB2, a hydration product of TxA2), measured by radioimmunoassay, were compared to those of corresponding healthy tissues. The production of 6-keto-PGF1a by endometrial cancer (20.8; 15.1-85.0 ng/mg protein/min, median and interquartile range), by healthy endometrium (25.5; 10.0-55.0), by healthy myometrium (34.9; 25.0-59.9) and by leiomyoma (20.3; 10.2-45.1) was similar. The production of TxB2 was increased by endometrial cancer (55.5; 10.5-155.2, p less than 0.02) in comparison with endometrium (9.8; 4.3-35.1), myometrium (3.8; 2.1-8.0) and leiomyoma (1.9; 1.0-3.8). The 6-keto-PGF1a/TxB2 ratio in endometrial cancer (0.9; 0.3-1.5) was smaller (p less than 0.02) than that in healthy endometrium (3.3; 1.9-4.8). Thus, TxA2 may be a factor in endometrial cancer.

Topics: 6-Ketoprostaglandin F1 alpha; Adult; Aged; Aged, 80 and over; Epoprostenol; Female; Humans; In Vitro Techniques; Leiomyoma; Middle Aged; Thromboxane A2; Thromboxane B2; Uterine Neoplasms

In order to investigate prostacyclin production by human myometrium, uterine cervix and leiomyoma, slices of each tissue were incubated. The 6-keto prostaglandin F1 alpha concentration of the incubation medium was measured by radioimmunoassay. The serosal and endometrial sides of myometrium produce 6-keto prostaglandin F1 alpha, with no significant difference in production between the two sides. The 6-keto PGF1 alpha production of leiomyoma was significantly higher than that of myometrium. Our results suggest that human myometrium and leiomyoma produce prostacyclin regardless of vascularization.

Topics: 6-Ketoprostaglandin F1 alpha; Cervix Uteri; Female; Humans; Kinetics; Leiomyoma; Mucous Membrane; Myometrium; Uterine Neoplasms

Prostaglandin release by leiomyomas obtained throughout the menstrual cycle was examined using a superfusion technique. The principal prostaglandin produced was 6-keto-PGF1 alpha, with PGF2 alpha and PGE2 in lesser amounts. No cyclical changes in prostaglandin release was detected.

Topics: 6-Ketoprostaglandin F1 alpha; Adult; Female; Humans; Leiomyoma; Menstruation; Middle Aged; Prostaglandins; Uterine Neoplasms