6-ketoprostaglandin-f1-alpha and Granuloma

Arachidonic acid (AA) injected locally into carrageenin/sponge granulomas, but not if given orally, inhibited granuloma growth. Granuloma macrophage (M0) infiltration was inhibited, and prostaglandin E2 (PGE2) synthesis (ng/100 mg granuloma dry weight) stimulated, by AA treatment. M0 adenosine 3',5'-cyclic monophosphate (cAMP) levels and granuloma exudate volume were not affected. Granuloma M0s incubated in vitro with arachidonic acid synthesised thromboxane B2 (Txb2), 6-ketoprostaglandin F1 alpha (6-ketoPGF1 alpha), and preferentially, PGE2. The AA inhibition of granuloma growth was possibly mediated via the synthesis of PGE2.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Anti-Inflammatory Agents; Arachidonic Acid; Arachidonic Acids; Carrageenan; Cyclic AMP; Dinoprostone; Eicosanoic Acids; Exudates and Transudates; Granuloma; Macrophages; Male; Prostaglandins E; Rats; Rats, Inbred Strains; Thromboxane B2

Seven-day-old carrageenin granuloma tissue of rats, when incubated in organ culture, produced 6-keto-prostaglandin F1 alpha, prostaglandin E2, prostaglandin F2 alpha, and thromboxane B2 in ratios of 16/7/1/1, respectively. Indomethacin (1 microgram/ml) inhibited this cyclooxygenase-mediated arachidonic acid metabolism. Dexamethasone (1 microgram/ml) also inhibited this metabolic cascade. Its inhibitory effect was not immediate; preincubation of the tissue with dexamethasone potentiated its inhibitory effectiveness. Cycloheximide (0.1 to 10 microgram/ml) also inhibited prostaglandin production. The inhibitory mechanism by cycloheximide of prostaglandin production was discussed in relation to that by dexamethasone.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Carrageenan; Culture Techniques; Cycloheximide; Dexamethasone; Granuloma; Indomethacin; Male; Prostaglandins; Prostaglandins E; Prostaglandins F; Rats; Thromboxane B2

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Carrageenan; Gas Chromatography-Mass Spectrometry; Granuloma; Humans; Ions; Male; Prostaglandins F; Rats

Levels of PGE2, PGF2 alpha, PGI2 (measured as 6-keto-PGF1 alpha), and thromboxane B2 were determined in rat inflammatory excuates induced 1, 3, and 7 days after carrageenin injection into air-pouch granuloma. The PGE2 and 6-keto-PGF1 alpha levels found in the exudate could not account for the differences in PGE2-like activity as measured by biologic and serologic methods.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Carrageenan; Exudates and Transudates; Granuloma; Inflammation; Male; Prostaglandins E; Prostaglandins F; Rats; Thromboxane B2; Thromboxanes; Time Factors