6-cyano-7-nitroquinoxaline-2-3-dione and Cardiovirus-Infections

Stabilizing the survival of oligodendrocytes and oligodendrocyte precursors within and near lesions in patients suffering from multiple sclerosis (MS) and other demyelinating diseases is an important therapeutic goal. Previous studies have identified a human-derived monoclonal IgM antibody designated rHIgM22 that induces remyelination in a mouse model of MS. We provide evidence that this antibody, directed against myelin, induces antiapoptotic signaling in premyelinating oligodendrocytes and reduces caspase-3 activation and caspase gene expression in mice undergoing antibody-induced remyelination. This effect was dependent on calcium entry via CNQX-sensitive channels and on lipid raft integrity, and was correlated with suppression of JNK signaling. We conclude that rHIgM22 may induce remyelination via rescue of oligodendrocytes, and suggest that such autoantibody-mediated signaling may have important therapeutic implications for a variety of neurological diseases, including stroke and Alzheimer's disease.

Topics: 6-Cyano-7-nitroquinoxaline-2,3-dione; Animals; Antibodies; Apoptosis; Calcium Channels; Cardiovirus Infections; Caspase 3; Caspases; Cell Line, Tumor; Cell Survival; Humans; Hydrogen Peroxide; JNK Mitogen-Activated Protein Kinases; Membrane Microdomains; Mice; Mitogen-Activated Protein Kinases; Multiple Sclerosis; Myelin Proteins; Myelin Sheath; Nerve Degeneration; Oligodendroglia; Rats; Signal Transduction; Stem Cells; Tumor Necrosis Factor-alpha