6-8-diprenylgenistein and Obesity

Obesity, which is characterized by excessive fat accumulation, is associated with several pathological disorders, including metabolic diseases. In this study, the anti-obesity effect of 6,8-diprenylgenistein (DPG), a major isoflavonoid of Cudrania tricuspidata fruits was investigated using high fat-diet (HFD)-induced obese mice at the doses of 10 and 30 mg/kg for six week. The body weight of the DPG-treated groups was significantly lower compared to the HFD-treated group. In addition, fat accumulation in epididymal adipose tissue and liver was dramatically decreased in the HFD + DPG groups. The food efficiency ratios of the HFD + DPG groups were also lower compared to the HFD group with the same food intake. Metabolic parameters that had increased in the HFD group were decreased in the HFD + DPG groups. Further studies demonstrate that DPG efficiently reduces lipogenic genes by regulation of transcription factors, such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and hormones, such as leptin and adiponection. DPG also regulates acetyl-CoA carboxylase (ACC) and hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) by AMP-activated protein kinase (AMPK) activation. Taken together, DPG is beneficial for the regulation of obesity, especially resulting from high fat intake.

Topics: Acetyl-CoA Carboxylase; Adiponectin; Adipose Tissue; AMP-Activated Protein Kinases; Animals; Anti-Obesity Agents; CCAAT-Enhancer-Binding Protein-alpha; Diet, High-Fat; Fruit; Gene Expression Regulation; Genistein; Hydroxymethylglutaryl CoA Reductases; Isoflavones; Leptin; Liver; Male; Mice; Mice, Inbred C57BL; Mice, Obese; Moraceae; Obesity; Plant Extracts; PPAR gamma