5-n-acetyl-9-(4-azidosalicoylamido)-2-deoxy-2-3-didehydroneuraminic-acid and Brain-Neoplasms

Several lines of evidence suggest that gangliosides may play a role in the regulation of growth in many cell types. Here we describe the effects on growth of two different cell lines by the addition of two different chemicals which have been reported to elevate the cellular ganglioside content through different mechanisms. Growth of neuroblastoma (Neuro 2a) cells in medium containing fetal bovine serum was inhibited in a dose-dependent fashion by both exogenous GM1 ganglioside and NeuAc2en, an inhibitor of sialidase activity. In contrast, growth of glioma cells (U-1242 MG) was not affected by exogenous GM1 or NeuAc2en in the presence of as little as 1% calf serum. However, NeuAc2en inhibited growth of U-1242 MG cells stimulated by platelet-derived growth factor in serum-free medium. These results demonstrate that the growth inhibitory effects of ganglioside on U-1242 MG but not Neuro 2a cells can be counteracted by serum, suggesting that the mechanisms through which gangliosides affect cell growth may be different for different growth factors and cell types.

Topics: Animals; Azides; Brain Neoplasms; Cell Division; Culture Media; Culture Media, Serum-Free; Depression, Chemical; G(M1) Ganglioside; Glioma; Humans; Mice; Neuraminidase; Neuroblastoma; Platelet-Derived Growth Factor; Sialic Acids; Tumor Cells, Cultured