5-9-12-octadecatrienoic-acid and Weight-Gain

In this study, we investigated effects of pine nut oil (PNO) on high-fat-diet (HFD)-induced obesity and metabolic dysfunction in skeletal muscle and brown adipose tissue (BAT). Male C57BL/6 mice were fed a HFD with 15% energy from lard and 30% energy from either soybean oil (SBO-HFD) or PNO (PNO-HFD) for 12 weeks. The PNO-HFD resulted in less weight gain and intramuscular lipid accumulation than the SBO-HFD and was accompanied by upregulation of transcripts and proteins related to oxidative metabolism and phosphorylation of AMP-activated protein kinase (AMPK), as well as molecules selectively expressed in type I and type IIa muscle fibers. In addition, uncoupling protein-1 was upregulated in BAT. These beneficial metabolic effects were partly associated with the dual ligand activity of pinolenic acid, which is abundant in PNO, for peroxisome proliferator-activated receptors α and δ. Our findings suggest that PNO may have potential as a dietary supplement for counteracting obesity and metabolic dysregulation.

Topics: Adipose Tissue, Brown; AMP-Activated Protein Kinases; Animals; Diet; Diet, High-Fat; Dietary Fats; Ion Channels; Linolenic Acids; Male; Mice; Mice, Inbred C57BL; Mitochondria; Mitochondrial Proteins; Muscle, Skeletal; Nuts; Obesity; Phosphorylation; Pinus; Plant Oils; PPAR alpha; PPAR delta; Soybean Oil; Thermogenesis; Uncoupling Protein 1; Weight Gain