5-22-cholestadien-3beta-ol and Disease-Models--Animal

The screen of 10 soft coral extracts collected from the Colombian Caribbean Sea in the TPA-induced ear edema model allowed us to identify Eunicea fusca extract among others as an interesting source of active compounds. The new diterpene, fuscoside E (1), along with the known fuscoside B (2), fuscol (3), (+)-germacrene D (4) and a mixture of six sterols (5-10), were isolated from this soft coral. Their structures were elucidated by 1D and 2D NMR spectroscopy techniques. Fuscoside E (1) absolute stereochemistry was determined by chiroptical methods. Fuscoside E (1) and B (2) showed strong anti-inflammatory in the above mentioned bioassay. Additionally, fuscoside E (1) and the sterol mixture (5-10) presented antifouling activity against bacterial strains involved in surface colonization.

Topics: Animals; Anthozoa; Anti-Bacterial Agents; Anti-Inflammatory Agents, Non-Steroidal; Bacillus; Biological Assay; Biological Products; Caribbean Region; Disease Models, Animal; Diterpenes; Drug Discovery; Drug Evaluation, Preclinical; Ear; Edema; Glycosides; Mice; Microbial Sensitivity Tests; Molecular Conformation; Molecular Structure; Stereoisomerism; Vibrio