5-10-15-20-tetra(4-hydroxyphenyl)porphyrin and Osteosarcoma

Photodynamic therapy (PDT) involves a photosensitizing agent activated with light to induce cell death. Two-photon excited PDT (TPE-PDT) offers numerous benefits compared to traditional one-photon induced PDT, including an increased penetration depth and precision. However, the in vitro profiling and comparison of two-photon photosensitizers (PS) are still troublesome. Herein, we report the development of an in vitro screening platform of TPE-PS using a 3D osteosarcoma cell culture. The platform was tested using three different two-photon (2P) active compounds - a 2P sensitizer P2CK, a fluorescent dye Eosin Y, and a porphyrin derivative (TPP). Their 2P absorption cross-sections (σ

Topics: Antineoplastic Agents; Benzylidene Compounds; Cell Line, Tumor; Cell Survival; Cisplatin; Eosine Yellowish-(YS); Humans; Mesenchymal Stem Cells; Osteosarcoma; Photons; Photosensitizing Agents; Porphyrins