5--methoxyhydnocarpin and Leukemia-P388

Bioassay (P388 lymphocytic leukemia cell line and human cancer cell lines) guided separation of an extract prepared from the leaves of Hymenaea palustris Ducké led to the isolation of six cancer cell growth inhibitory flavonoids (1-6). The structures were elucidated by HRMS and 1D and 2D NMR spectral analysis. The new flavonolignan 1 designated palstatin proved to be a methoxy structural modification of 5'-methoxyhydnocarpin-D (2). Flavones 1-4 inhibited growth of the pathogenic bacteria Enterococcus faecalis and/or Neisseria gonorrhoeae.

Topics: Animals; Anti-Bacterial Agents; Antineoplastic Agents, Phytogenic; Drug Screening Assays, Antitumor; Enterococcus faecalis; Fabaceae; Flavonoids; Humans; Inhibitory Concentration 50; Leukemia P388; Mice; Microbial Sensitivity Tests; Molecular Conformation; Molecular Structure; Neisseria gonorrhoeae; Nuclear Magnetic Resonance, Biomolecular; Peru; Plant Leaves; Trees; Tumor Cells, Cultured