5-(4-phenylbutoxy)psoralen and Hepatitis-C

Virus entry into cells is the initial stage of infection and involves multiple steps, and interfering viral entry represents potential antiviral approaches. Ion channels are pore-forming membrane proteins controlling cellular ion homeostasis and regulating many physiological processes, but their roles during viral infection have rarely been explored. Here, the functional Kv1.3 ion channel was found to be expressed in human hepatic cells and tissues. The Kv1.3 was then revealed to restrict HCV entry via inhibiting endosome acidification-mediated viral membrane fusion. The Kv1.3 was also demonstrated to inhibit DENV and ZIKV with an endosome acidification-dependent entry, but have no effect on SeV with a neutral pH penetration. A Kv1.3 antagonist PAP-1 treatment accelerated animal death in ZIKV-infected Ifnar1

Topics: Animals; Chlorocebus aethiops; Dengue; Dengue Virus; Endosomes; Ficusin; HEK293 Cells; Hepacivirus; Hepatitis C; Humans; Hydrogen-Ion Concentration; Kv1.3 Potassium Channel; Mice; Mice, Inbred C57BL; Mice, Knockout; Respirovirus Infections; Sendai virus; Transfection; Vero Cells; Virus Internalization; Zika Virus; Zika Virus Infection