5-(4-methoxybenzylidene)thiazolidine-2-4-dione and Acute-Disease

To test the effect of 5-(4-methoxybenzylidene)-2-thioxo-dihydropyrimidine-4, 6 (1H, 5H)-dione (SKLB-102) on acute hepatic inflammatory induced by concanavalin A (ConA) in mice.. The inhibitive effect of SKLB-102 on RAW264.7 cell migration induced by recombinant rat monocyte chemotactic protein-1 (MCP-1) was tested. The serum from the ConA-treated mice was collected after intragastric administration of SKLB-102 at the dose of 50 mg/kg bodyweight. The serum AST and ALT were determined by an automatic analyzer, and the serum TNF-alpha was determined with enzyme-linked immunosorbent assay (ELISA) kits. The liver samples were fixed in 10% formalin, embedded in paraffin, sectioned and then stained with hematoxylin and eosin for histological examinations.. SKLB-102 markedly reduced cell migrations, successfully reduced serum AST, ALT and down-regulated TNF-alpha.. SKLB-102 is likely to suppress the occurrence of Con A-induced hepatitis by suppressing macrophages migration and TNF-alpha releases.

Topics: Acute Disease; Animals; Cell Line; Cell Movement; Chemical and Drug Induced Liver Injury; Concanavalin A; Liver; Macrophages; Male; Mice; Mice, Inbred BALB C; Random Allocation; Thiazolidinediones