4-thiouridine-triphosphate has been researched along with Acquired-Immunodeficiency-Syndrome* in 1 studies
1 other study(ies) available for 4-thiouridine-triphosphate and Acquired-Immunodeficiency-Syndrome
Article | Year |
---|---|
Active site labeling of HIV-1 reverse transcriptase.
The human immunodeficiency virus-1 reverse transcriptase (HIV-1 RT) heterodimer (M(r) = 66,000 and M(r) = 51,000) has been photoaffinity labeled using 4-thiodeoxyuridine triphosphate (S4-dUTP) as a probe. A nascent polymerization complex was assembled from a single-stranded DNA template, a 12-mer DNA primer, and the necessary dNTPs (one of which was alpha-32P-labeled) to extend the primer to produce the n-1 product. The photoaffinity probe was then uniquely added at the 3'-terminal position of the extended primer bound at the catalytic site and photolyzed. The larger subunit (p66) was exclusively derivatized. The unique radioactive peptide resulting from proteolysis was isolated and identified by amino acid sequencing. Topics: Acquired Immunodeficiency Syndrome; Affinity Labels; Amino Acid Sequence; Base Sequence; Binding Sites; DNA; HIV Reverse Transcriptase; HIV-1; Molecular Sequence Data; RNA-Directed DNA Polymerase; Sequence Analysis; Uridine Triphosphate | 1993 |