4-hydroxyderricin and Hyperglycemia

Glucose uptake in skeletal muscle is crucial for glucose homeostasis.. Insulin and muscle contraction increase glucose uptake accompanied by the translocation of glucose transporter (GLUT) 4. In a search for promising foods, which can increase glucose uptake in skeletal muscle, we screened for active polyphenols by assaying for uptake of 2-deoxyglucose (2DG) in rat L6 muscle cells. Among 37 compounds, 4-hydroxyderricin and xanthoangelol, prenylated chalcones abundant in Ashitaba (Angelica keiskei Koidzumi, family Apiaceae), significantly increased 2DG uptake in L6 cells by 1.9-fold at 10 μM, compared with the level in DMSO-treated control cells. Next, we investigated the effect of these chalcones on the translocation of GLUT4 and its underlying mechanisms. The chalcones increased the GLUT4 level in the plasma membrane of L6 cells, but activated neither protein kinase C ζ/λ, Akt, nor adenosine monophosphate-activated protein kinase, all of which regulate the GLUT4 translocation. Interestingly, the oral administration of a titrated chalcone-enriched Ashitaba extract containing 150.6 mg/g (dry base) of 4-hydroxyderricin and 146.0 mg/g (dry base) of xanthoangelol suppressed acute hyperglycemia in oral glucose tolerance tests of mice.. Ashitaba is a promising functional food for the maintenance of the blood glucose level by inducing skeletal muscle-associated glucose uptake.

Topics: Angelica; Animals; Cell Line; Chalcone; Deoxyglucose; Flavonoids; Glucose; Glucose Tolerance Test; Glucose Transporter Type 4; Hyperglycemia; Male; Mice; Mice, Inbred ICR; Muscle Fibers, Skeletal; Muscle Proteins; Muscle, Skeletal; Phenols; Polyphenols; Prenylation; Protein Kinase C; Protein Transport; Random Allocation; Rats