4-hydroxy-2-nonenal and Helminthiasis--Animal

The aim of this paper is to assess the antioxidant properties of rat liver in the course of acute and chronic fasciolosis. Wistar rats were infected per os with 30 metacercariae of Fasciola hepatica. Liver activities of antioxidant enzymes and concentrations of non-enzymatic antioxidants were determined at 4, 7, and 10 weeks post-infection. Activities of superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GSH-Px), and glutathione reductase (GSSG-R) were decreased, catalase (CAT) activity was increased and non-enzymatic antioxidant concentrations (reduced glutathione, vitamins C, E and A) were reduced simultaneously with enhancement of lipid peroxidation processes as evidenced by increased levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). Changes in the antioxidant abilities of the liver and in the phospholipid structure of the cell membrane were accompanied by rising activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as markers of liver damage.

Topics: Alanine Transaminase; Aldehydes; Animals; Antioxidants; Aspartate Aminotransferases; Catalase; Disease Models, Animal; Enzymes; Fasciola hepatica; Glutathione Peroxidase; Glutathione Reductase; Helminthiasis, Animal; Lipid Peroxidation; Liver; Male; Malondialdehyde; Parasitic Diseases, Animal; Rats; Rats, Wistar; Superoxide Dismutase