4-6-dimorpholino-n-(4-nitrophenyl)-1-3-5-triazin-2-amine and Coronavirus-Infections

PEDV remains one of the most important swine diseases that infects pigs of all ages. It causes devastating viral enteric disease in piglets with a high mortality rate, leading to significant threats and huge economic loss to the pork industry. In this study, a transcriptomic shotgun sequencing (RNA-Seq) procedure was used to study gene responses against PEDV infection. Genome-wide analysis of differentially expressed genes (DEGs) was performed in Vero E6 cells post-PEDV infection. mTOR signaling pathway activator-MHY1485, and inhibitor-PP242 were used to study the antiviral function. Results revealed that the IRF3 was significantly up-regulated post-PEDV infection. Although most of the IFN-regulatory and -related genes evaluated in this study were either down-regulated or remained unchanged, IL11 behaved significantly up-regulated, with the peak at 16 hpi. Nearly 90% of PEDV infections were suppressed in the PP242 pretreated cells whereas the reverse effect was observed in the MYH1485 pretreated cells. Results indicated that the mTOR signaling pathway played a vital role in the PEDV antiviral regulation in the Vero E6 cells. Future studies will contribute to better understand the cellular antiviral mechanism against PEDV.

Topics: Animals; Antiviral Agents; Chlorocebus aethiops; Coronavirus Infections; Down-Regulation; Gene Expression; Gene Expression Profiling; Indoles; Interleukin-11; Morpholines; Porcine epidemic diarrhea virus; Proteome; Proteomics; Purines; Signal Transduction; Swine; Swine Diseases; Transcriptome; Triazines; Vero Cells; Virus Replication