4-4-difluoro-4-bora-3a-4a-diaza-s-indacene and Obesity

To investigate the effect of obesity induced by high fat diet on decidual reaction of endometrium in mice, and the effect of high fat treatment on decidual reaction of endometrial stromal cells.. Twelve 4-week-old healthy C57BL/6J female mice were randomly divided into high fat diet group and control group with 6 mice in each group. They were fed with high fat diet (22 kJ/g) or normal diet (16 kJ/g) for 12 weeks, respectively. The body weight of mice was measured every week. After feeding for 12 weeks, the body length and width of mice were measured, and the levels of fasting serum triglyceride and total cholesterol were determined. Then the mice were mated with healthy C57BL/6J male mice, and the uterine tissues were collected on the seventh day of pregnancy. The decidual cells and collagen fibers in mouse endometrium was observed by HE staining and Masson staining respectively. The expression of decidual reaction related proteins in mouse endometrium were detected by immunohistochemistry and Western blotting. Mouse endometrial stromal cells (mESCs) were isolated and treated with the oleic acid and palmitic acid. After feeding for 12 weeks, the body weight of mice in the high fat group was significantly higher than that in the control group (. Obesity induced by high fat diet and high fat treatment can impair the decidual reaction of endometrium and endometrial stromal cells in mice.

Topics: Animals; Azo Compounds; Body Weight; Bone Morphogenetic Proteins; Boron Compounds; Cholesterol; Collagen; Diet, High-Fat; Endometrium; Estradiol; Female; Homeobox A10 Proteins; Male; Mice; Mice, Inbred C57BL; Obesity; Oleic Acid; Palmitic Acid; Phalloidine; Pregnancy; Progesterone; Prostaglandin-Endoperoxide Synthases; Triglycerides

The proportions of body fat and fat-free mass are determining factors of adiposity-associated diseases. Work in Caenorhabditis elegans has revealed evolutionarily conserved pathways of fat metabolism. Nevertheless, analysis of body composition and fat distribution in the nematodes has only been partially unraveled because of methodological difficulties. We characterized metabolic C. elegans mutants by using novel and feasible BODIPY 493/503-based fat staining and flow cytometry approaches. Fixative as well as vital BODIPY staining procedures visualize major fat stores, preserve native lipid droplet morphology, and allow quantification of fat content per body volume of individual worms. Colocalization studies using coherent anti-Stokes Raman scattering microscopy, Raman microspectroscopy, and imaging of lysosome-related organelles as well as biochemical measurement confirm our approaches. We found that the fat-to-volume ratio of dietary restriction, TGF-β, and germline mutants are specific for each strain. In contrast, the proportion of fat-free mass is constant between the mutants, although their volumes differ by a factor of 3. Our approaches enable sensitive, accurate, and high-throughput assessment of adiposity in large C. elegans populations at a single-worm level.

Topics: Adipose Tissue; Adiposity; Animals; Azo Compounds; Boron Compounds; Caenorhabditis elegans; Disease Models, Animal; Fixatives; Flow Cytometry; Fluorescence; Germ-Line Mutation; High-Throughput Screening Assays; Lipid Metabolism; Microscopy; Obesity; Species Specificity; Spectrum Analysis, Raman; Staining and Labeling; Transforming Growth Factor beta