4-4-difluoro-4-bora-3a-4a-diaza-s-indacene and Antiphospholipid-Syndrome

4-4-difluoro-4-bora-3a-4a-diaza-s-indacene has been researched along with Antiphospholipid-Syndrome* in 1 studies

Other Studies

1 other study(ies) available for 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene and Antiphospholipid-Syndrome

ArticleYear
Phagocytosis of platelet microvesicles and beta2- glycoprotein I.
    Thrombosis and haemostasis, 2010, Volume: 104, Issue:2

    The majority of the antiphospholipid antibodies, present in patients with antiphospholipid syndrome, are directed against conformational epitopes in beta2-glycoprotein I. beta2-glycoprotein I is an anionic phospholipid-binding 50-kDa plasma protein whose physiological role is not clear. Here we investigate the role of beta2-glycoprotein I in the phagocytosis of phosphatidylserine-expressing platelet microvesicles and the effect of autoantibodies to beta2-glycoprotein I on this process. We labelled the glycans of beta2-glycoprotein I with BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)-hydrazide without affecting its phospholipid binding capacity. BODIPY-beta2-glycoprotein I bound to platelet microvesicles in a concentration-dependent manner and promoted the phagocytosis of platelet microvesicles by THP-1 derived macrophages in vitro at physiological plasma concentrations with a half maximal effect at approximately 10 microg/ml. beta2-glycoprotein I-stimulated phagocytosis was inhibited by annexin A5 and the phosphatidylserine-binding C1C2 fragment of lactadherin. Furthermore, immunoaffinity purified beta2-glycoprotein I-dependent antiphospholipid antibodies from five patients with antiphospholipid syndrome inhibited the phagocytosis in a concentration-dependent manner. These studies suggest that the binding of beta2-glycoprotein I to phosphatidylserine-expressing procoagulant platelet microvesicles may promote their clearance by phagocytosis and autoantibodies to beta2-glycoprotein I may inhibit this process to induce a procoagulant state.

    Topics: Annexin A5; Antigens, Surface; Antiphospholipid Syndrome; Autoantibodies; beta 2-Glycoprotein I; Blood Coagulation; Blood Platelets; Boron Compounds; Cell Line; Cytoplasmic Vesicles; Fluorescent Dyes; Humans; Macrophages; Milk Proteins; Peptide Fragments; Phagocytosis; Phosphatidylserines

2010