4--7-dihydroxyflavone and Leishmaniasis

The treatment for leishmaniasis is currently plagued by side effects such as toxicity and the emergence of drug resistance to the available repertoire of drugs, as well as the expense of these drugs. Considering such rising concerns, we report the anti-leishmanial activity and mechanism of a flavone compound 4',7-dihydroxyflavone (TI 4). Four flavanoids were initially screened for anti-leishmanial activity and cytotoxicity. The results showed that the compound TI 4 exhibited higher activity and selectivity index at the same time as maintaining low cytotoxicity. Preliminary microscopic studies and fluorescence-activated cell sorting analysis reported that the parasite underwent apoptosis on TI 4 treatment. Further in-depth studies revealed high reactive oxygen species (ROS) production and thiol levels in the parasites, suggesting ROS-mediated apoptosis in the parasites upon TI 4 treatment. Other apoptotic indicators such as intracellular Ca

Topics: Animals; Antiprotozoal Agents; Apoptosis; Leishmania; Leishmaniasis; Parasites; Reactive Oxygen Species

Topics: Animals; Antiprotozoal Agents; Leishmania donovani; Leishmaniasis; Nanotubes, Carbon; Parasites; Reactive Oxygen Species