3alpha-hydroxy-4alpha-methylergosta-8-24(28)-dien-7-11-dione-26-oic-acid and Leukemia-P388

Three new steroids, zhankuic acids A [1], B [2], and C [3], were isolated from the fruiting bodies of Antrodia cinnamomea by bioassay-guided fractionation. The structures of these compounds were elucidated by chemical reactions and detailed analysis of their 1H- and 13C-nmr spectra. Biological studies revealed that 1 exhibited cytotoxic activity against P-388 murine leukemia cells and 2 showed weak anticholinergic and antiserotonergic activities.

Topics: Animals; Antineoplastic Agents; Cholinergic Antagonists; Crystallization; Ergosterol; Gas Chromatography-Mass Spectrometry; Guinea Pigs; Ileum; Leukemia P388; Magnetic Resonance Spectroscopy; Male; Medicine, Chinese Traditional; Muscle Contraction; Muscle Relaxation; Muscle, Smooth; Polyporaceae; Serotonin Antagonists; Tumor Cells, Cultured