3-nitrotyrosine has been researched along with Neurofibromatosis-1* in 2 studies
2 other study(ies) available for 3-nitrotyrosine and Neurofibromatosis-1
| Article | Year |
|---|---|
Cell culture study on neurofibromatosis.
Cells obtained on culture from normal appearing skin (NFns) and from neurofibromas of neurofibromatosis patients (NFnf), and from normal skin of normal donors (Normal) were studied. We measured the growth rate, maximal cell density, radiation sensitivity and resistance to 3-nitrotyrosine of each of the three groups of strains. The growth rate and maximal cell density of fibroblasts derived from neurofibromas of the neurofibromatosis patients were significantly lower than those in normal donors. The neurofibromatosis cells showed no X-ray hypersensitivity, as compared to normal controls. We also did not observe resistance to 3-nitrotyrosine of the cells derived from normal appearing skin of the neurofibromatosis patients. However, cells derived from neurofibromas of the neurofibromatosis patients showed resistance to 3-nitrotyrosine. This phenomenon may explain the slow growth rate of the latter cells. Topics: Cell Count; Cell Division; Cells, Cultured; Humans; Neurofibromatosis 1; Radiography; Skin; Tyrosine | 1987 |
The pathophysiology of neurofibromatosis. I. Resistance in vitro to 3-nitrotyrosine as an expression of the mutation.
The in vitro expression of the autosomal dominant mutation responsible for neurofibromatosis was probed using the amino acid analogue 3-nitrotyrosine as a cell culture selective agent. The presence of 3-nitrotyrosine in culture medium led to inhibition of growth and cell death among normal skin fibroblasts in log phase growth, whereas cell strains derived from six different patients' neurofibromas or skin cells, or both, exhibited a consistently enhanced ability to survive under the same conditions. At 0.8 mM 3-nitrotyrosine, four patient-derived skin fibroblast strains could be differentiated from five strains of control skin fibroblasts with a high level of confidence (P < 0.0000). In the same way four neurofibroma-derived fibroblast strains were differentiated from control skin fibroblasts (P < 0.0022). Neurofibroma-derived cells were not different from control cells when treated with 5-fluorotryptophan or p-fluorophenylalanine. Topics: Adolescent; Adult; Cell Division; Cell Survival; Cells, Cultured; Child; Child, Preschool; Dose-Response Relationship, Drug; Drug Resistance; Female; Fibroblasts; Humans; Infant, Newborn; Male; Middle Aged; Neurofibroma; Neurofibromatosis 1; Skin; Tyrosine | 1980 |