3-nitrotyrosine and Neointima
3-nitrotyrosine has been researched along with Neointima* in 2 studies
Other Studies
2 other study(ies) available for 3-nitrotyrosine and Neointima
Article | Year |
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The pro-atherogenic response to disturbed blood flow is increased by a western diet, but not by old age.
Atherogenic remodeling often occurs at arterial locations with disturbed blood flow (i.e., low or oscillatory) and both aging and western diet (WD) increase the likelihood for pro-atherogenic remodeling. However, it is unknown if old age and/or a WD modify the pro-atherogenic response to disturbed blood flow. We induced disturbed blood flow by partial carotid ligation (PCL) of the left carotid artery in young and old, normal chow (NC) or WD fed male B6D2F1 mice. Three weeks post-PCL, ligated carotid arteries had greater intima media thickness, neointima formation, and macrophage content compared with un-ligated arteries. WD led to greater remodeling and macrophage content in the ligated artery compared with NC mice, but these outcomes were similar between young and old mice. In contrast, nitrotyrosine content, a marker of oxidative stress, did not differ between WD and NC fed mice, but was greater in old compared with young mice in both ligated and un-ligated carotid arteries. In primary vascular smooth muscle cells, aging reduced proliferation, whereas conditioned media from fatty acid treated endothelial cells increased proliferation. Taken together, these findings suggest that the remodeling and pro-inflammatory response to disturbed blood flow is increased by WD, but is not increased by aging. Topics: Aging; Animals; Atherosclerosis; Carotid Arteries; Carotid Intima-Media Thickness; Cell Proliferation; Diet, Western; Endothelial Cells; Fatty Acids; Male; Mice; Muscle, Smooth, Vascular; Myocytes, Smooth Muscle; Neointima; Oxidative Stress; Regional Blood Flow; Tyrosine | 2019 |
Increased Oxidative Stress and Hypoxia Inducible Factor-1 Expression during Arteriovenous Fistula Maturation.
The poor clinical results that are frequently reported for arteriovenous fistulae (AVF) for hemodialysis are typically due to failure of AVF maturation. We hypothesized that early AVF maturation is associated with generation of reactive oxygen species and activation of the hypoxia-inducible factor-1 (HIF-1) pathway, potentially promoting neointimal hyperplasia. We tested this hypothesis using a previously reported mouse AVF model that recapitulates human AVF maturation.. Aortocaval fistulae were created in C57Bl/6 mice and compared with sham-operated mice. AVFs or inferior vena cavas were analyzed using a microarray, Amplex Red for extracellular H. Oxidative stress was higher in AVF than that in control veins, with more H. Oxidative stress increases in mouse AVF during early maturation, with increased expression of HIF-1α and its target genes NOX-2, HO-1, and VEGF-A. These results suggest that clinical strategies to improve AVF maturation could target the HIF-1 pathway. Topics: Animals; Aorta; Arteriovenous Shunt, Surgical; Gene Expression Regulation; Heme Oxygenase-1; Hydrogen Peroxide; Hyperplasia; Hypoxia-Inducible Factor 1, alpha Subunit; Male; Membrane Proteins; Mice, Inbred C57BL; NADPH Oxidase 2; Neointima; Oxidative Stress; Reactive Oxygen Species; Signal Transduction; Time Factors; Tyrosine; Vascular Endothelial Growth Factor A; Vascular Patency; Vena Cava, Inferior | 2017 |