3-carbamoyl-2-2-5-5-tetramethyl-1-pyrrolidinyl-n-oxyl and Obesity

In vivo imaging of oxidative stress can facilitate the understanding and treatment of cardiovascular diseases. We evaluated nitroxide-enhanced MRI with 3-carbamoyl-proxyl (3CP) for the detection of myocardial oxidative stress.. Three mouse models of cardiac oxidative stress were imaged, namely angiotensin II (Ang II) infusion, myocardial infarction (MI), and high-fat high-sucrose (HFHS) diet-induced obesity (DIO). For the Ang II model, mice underwent MRI at baseline and after 7 days of Ang II (n = 8) or saline infusion (n = 8). For the MI model, mice underwent MRI at baseline (n = 10) and at 1 (n = 8), 4 (n = 9), and 21 (n = 8) days after MI. For the HFHS-DIO model, mice underwent MRI at baseline (n = 20) and 18 weeks (n = 13) after diet initiation. The 3CP reduction rate, K. For the Ang II model, K. Nitroxide-enhanced MRI noninvasively quantifies tissue oxidative stress as one component of a multiparametric preclinical MRI examination. These methods may facilitate investigations of oxidative stress in cardiovascular disease and related therapies.

Topics: Adenosine; Angiotensin II; Animals; Cardiovascular System; Cyclic N-Oxides; Diet, High-Fat; Dietary Sucrose; Disease Models, Animal; Magnetic Resonance Imaging; Male; Mice, Inbred C57BL; Myocardial Infarction; Nitrogen Oxides; Obesity; Oxidative Stress; Perfusion; Pyrrolidines