3-bromoacetylamino-benzoylurea and Leukemia--T-Cell

3-bromoacetylamino-benzoylurea has been researched along with Leukemia--T-Cell* in 1 studies

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1 other study(ies) available for 3-bromoacetylamino-benzoylurea and Leukemia--T-Cell

ArticleYear
3-Bromoacetylamino benzoylurea (3-BAABU), a new antimicrotubule cancericidal agent applied in cytogenetic analysis in hematology.
    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 1998, Volume: 52, Issue:6

    3-Bromoacetylamino benzoylurea (3-BAABU) is a newly synthesized antimicrotubule cancericidal compound. In the present study, we investigated the possibility of using 3-BAABU as a mitotic blocking agent for hematologic karyotyping. Treatment with 3-BAABU caused scattering of metaphase chromosomes throughout the cytoplasm both in phytohemagglutinine (PHA)-stimulated human lymphocytes and in human leukemic cells. Kinetic showed a rapid uptake of 3-BAABU by treated cells and irreversibility of its effect. Using 3-BAABU in routine procedure, a karyotype of lymphocytes from a normal male was 46, XY, with normal structure and CEM leukemic line was 85, XX, in a representative spread with abnormalities similar to reports using other blocking agents. Using 3-BAABU in spectral karyotyping, details of translocations in CEM leukemic cells were readily detected in several chromosomes, such as 7 [t(7;11)], 8 [t(8;9)], 9 [t(8;9) & t(9;19)], 11 [t(7;11)], 16 [t(16;18;20)] and 20 [t(1;20)]. 3-BAABU displayed two important characters in cytogenetics, 1) it caused dispersion of chromosomes, avoiding chance of overlap; 2) compared to the conventional mitotic blocking agent, vinblastine sulfate, 3-BAABU exhibited much gentle effect on chromosomes, thus providing more flexibility in time to perform karyotyping.

    Topics: Antineoplastic Agents; Chromosomes, Human; Cytogenetics; DNA Fragmentation; Humans; Karyotyping; Kinetics; Leukemia, T-Cell; Lymphocyte Activation; Lymphocytes; Male; Microtubules; Mitosis; Translocation, Genetic; Tumor Cells, Cultured; Urea

1998