3-5-bis(2-fluorobenzylidene)piperidin-4-one and Triple-Negative-Breast-Neoplasms

EF24, a curcumin analog, exerts a potent antitumor effect on various cancers. However, whether EF24 retards the progression of triple-negative breast cancer (TNBC) remains unclear. In this study, we explored the role of EF24 in TNBC and clarified the underlying mechanism. In a mouse model of TNBC xenograft, EF24 administration reduced the tumor volume, suppressed cell proliferation, promoted cell apoptosis, and downregulated long noncoding RNA human leukocyte antigen complex group 11 (HCG11) expression. In TNBC cell lines, EF24 administration reduced cell viability, suppressed cell invasion, and downregulated HCG11 expression. HCG11 overexpression reenhanced the proliferation and invasion of TNBC cell lines suppressed by EF24. The following mechanism research revealed that HCG11 overexpression elevated Sp1 transcription factor (Sp1) expression by reducing its ubiquitination, thereby enhanced Sp1-mediated cell survival and invasion in the TNBC cell line. Finally, the

Topics: Animals; Benzylidene Compounds; Cell Movement; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; Mice; MicroRNAs; Piperidones; RNA, Long Noncoding; Sp1 Transcription Factor; Triple Negative Breast Neoplasms; Xenograft Model Antitumor Assays